Mammalian Cell Transfections
Utilizing the ECM 2001+ System Complete, for Cuvettes (452046) for standard mammalian cell transfections is simple. The AC feature is turned off and the DC mode is set as a square wave electroporation device. The range of voltages and pulse lengths that can be programmed, coupled with the ability to carry out up to 10 pulses per experiment, make this an extremely versatile system for any lab.
ECM 2001+ Generator, 630B Safety Stand, Cuvettes 1 mm, 2 mm, 4 mm, pkg. of 30 (10 each) and Cuvette Rack
The ECM 2001+ Electrofusion and Electroporation System provides fast, efficient cell fusion in hybridoma production, hybrid cell formation and nuclear transfer applications, as well as square wave electroporation for transfecting genes and other molecules into mammalian cell lines.
ECM 2001 GENERATOR
The ECM 2001+ is a multifunctional electrofusion and square wave electroporation generator. The ability to generate both AC and DC waves allows for fast and efficient cell fusion in hybridoma production, hybrid cell formation, and nuclear transfer applications. This system is powerful enough to yield high transfection efficiencies for cell lines and difficult to transfect cell types including stem cells and primary cells. The gentle square wave pulse also allows for high cell viability of these cell types.
The ECM 2001+ is intended For Research Use Only. Not for use in diagnostic, pre-clinical, or clinical procedures.
AC sine wave aligns cells by dielectrophoresis for electrofusion applications. Square DC waveform provides the fusion pulse for electrofusion or is utilized in mammalian electroporation applications.
FEATURES & BENEFITS
- AC waveform of 0.2 – 2.0 MHz
- Square wave electroporation capabilities
- A wide range of voltages from 5 V to 3000 V
- Fine voltage discrimination
- Advanced programming capability to combine multiple AC steps pre- and post-DC fusion step
- Capable of operating at low impedance loads
- Large touchscreen interface
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