ECM 2001+ EMBRYO MANIPULATION SYSTEM – Witec AG

Embryo Manipulation

For use in small volume cell fusion applications such as embryo manipulation, we recommend the ECM 2001+ Embryo Manipulation System (452047).

Description

Included items:
In this system the ECM 2001 generator, 0.5 mm gap, 1 mm gap, and 3.2 mm gap microslides, and micrograbber adaper cables are provided.

The ECM 2001+ Electrofusion and Electroporation System provides fast, efficient cell fusion in hybridoma production, hybrid cell formation and nuclear transfer applications, as well as square wave electroporation for transfecting genes and other molecules into mammalian cell lines.

ECM 2001 GENERATOR

The ECM 2001+ is a multifunctional electrofusion and square wave electroporation generator. The ability to generate both AC and DC waves allows for fast and efficient cell fusion in hybridoma production, hybrid cell formation, and nuclear transfer applications. This system is powerful enough to yield high transfection efficiencies for cell lines and difficult to transfect cell types including stem cells and primary cells. The gentle square wave pulse also allows for high cell viability of these cell types.

The ECM 2001+ is intended For Research Use Only. Not for use in diagnostic, pre-clinical, or clinical procedures.

WAVEFORMS

AC sine wave aligns cells by dielectrophoresis for electrofusion applications. Square DC waveform provides the fusion pulse for electrofusion or is utilized in mammalian electroporation applications.

FEATURES & BENEFITS

  • AC waveform of 0.2 – 2.0 MHz
  • Square wave electroporation capabilities
  • A wide range of voltages from 5 V to 3000 V
  • Fine voltage discrimination
  • Advanced programming capability to combine multiple AC steps pre- and post-DC fusion step
  • Capable of operating at low impedance loads
  • Large touchscreen interface

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