WesternBright ECL - HRP Substrate

Art No | K-12045

WesternBright ECL - HRP Substrate

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from 41.00 CHF

WesternBright ECL requires as little as ten times less antibody than other substrates, allowing you to conserve precious antibodies and samples.

Description

WesternBright ECL is a horseradish peroxidase substrate. It is more sensitive than other chemiluminescent substrates and uses up to 10 times less antibody than other substrates, allowing you to save precious antibodies and samples. Additionally, the WesternBright ECL signal is long lasting, allowing multiple exposures without substantial signal decay.

The best choice for routine, chemiluminescent Western blots:

Stronger signal than Amersham™ ECL or Pierce™ ECL
Sensitive – detect low pg protein amounts
Lasting signal – up to 6 hrs
Save money – use up to 10x less antibody

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500ml kit- contains substrate for 5000cm2 membrane

Includes:
WesternBright ECL Luminol/enhancer solution
WesternBright Peroxide Chemiluminescent peroxide solution

WesternBright ECL is optimized for chemiluminescent Western blots.

WesternBright ECL is specially formulated to produce a strong signal for sensitive film imaging. WesternBright ECL substrate produces a strong, long-lasting signal after reaction with HRP. Duplicate slot blots containing serial dilutions of transferrin protein were detected using WesternBright ECL or Amersham™ ECL™ (GE Healthcare). Both blots were simultaneously exposed to the same sheet of film for 15 seconds. WesternBright ECL is several times more sensitive than Amersham ECL.

Westernbright ECL has high sensitivity for detection of low-abundance proteins.

The higher sensitivity of WesternBright ECL is especially important when detecting low abundance proteins. Duplicate Western blots with serial dilutions of HeLa cell lysate were probed for ERK1 and detected using either WesternBright ECL or Amersham ECL HRP substrates. Each blot was exposed to film for 1 minute.

WesternBright ECL requires up to ten times less antibody than other substrates.

Duplicate slot blots containing serial dilutions of transferrin were probed with the antibody diutions shown, and detected with either Westernbright ECL or Pierce ECL (Thermo Scientific) according to the manufacturer’s instructions. The blots were imaged simultaneously on the same piece of film. WesternBright ECL produced the same sensitivity with ten times less primary and five times less secondary antibody.