Size: 250µl
Fluorescently labeled secondary antibodies
Description
Advansta offers a broad selection of fluorescently labeled secondary antibodies with a variety of conjugate types, excitable with both visible and near infrared (NIR) light. Choose the label and species best suited for your application. Each high quality antibody offers unparalleled sensitivity and performance for immunoblotting applications. Additionally, achieve fast and convenient workflow when using the dyes in conjunction with Advansta's WesternBright™ MCF system for multicolor fluorescent Westerns.
- Fluorescent detection –secondary antibodies with a range of fluorescent conjugates
- Performance - strong signal and no cross-reactivity in immunoblotting applications
- Convenience - each antibody is demonstrated to provide optimal results with Advansta's Western blotting system
- Flexibility - choose from visible or near IR fluorescent dyes and target species best suited to your application
Excitation maximum (nm) | Emission maximum (nm) | |
Dye-490 | 491 | 515 |
Dye-550 | 551 | 565 |
Dye-650 | 653 | 672 |
Dye-IR700 | 690 | 709 |
Dye-IR800 | 783 | 800 |
Each high quality antibody offers unparalleled sensitivity and performance. Choose from secondary antibodies of different species specificities conjugated with fluorophores in the visible or near-infrared (IR) range.
SpectraDye secondary antibodies demonstrate sensitivity and performance. Purified transferrin was spiked into increasing amounts of HeLa cell lysate. The Western blot was probed with primary antibodies Rabbit anti-transferrin, rat anti-tubulin, and mouse anti-GAPDH; secondary antibodies were anti-rabbit SpectraDye 650 (red), anti-rat SpectraDye 550 (green), and anti-mouse SpectraDye 490 (blue).