FUSION ABSOLUTE - Western Blot & Gel Documentation Imaging – Witec AG

ABSOLUTE QUANTIFICATION
Absolute quantification allows researchers to compare and integrate data from different studies or laboratories. Unlike relative quantification, which compares the amount of an analyte between two or more samples, absolute quantification provides a precise value for the amount of substance in the sample, expressed in absolute units.

ABSOLUTE CALIBRATION
Absolute NIST Traceable Calibration Imaging is based on traceable references provided by the National Institute of Standards and Technology (NIST). It allows precise quantification of specific biomolecules (such as proteins) in the sample of interest and provides benefits such as accuracy, reproducibility, and standardization.

ABSOLUTE TIME SAVER
Once your sample is placed in the machine, the system evaluates the required camera positioning distance. The Z-axis moves automatically according to the nature and size of the sample and suggests automatic settings. Designed to avoid time-consuming manipulation, our new system also features a wide-opening door so you can easily arrange your samples.

ABSOLUTE FLEXIBILITY
You are used to working on computer-based systems? The nondisplay version of the FUSION ABSOLUTE offers similar performance in a compact format. Depending on your needs and applications you can also customize your system. Finally, another innovative feature that comes with the FUSION ABSOLUTE is its new UV-LED Pad for your fluorescence experiments.

FOR UNRIVALLED DETECTION

Designed to exceed your expectations, the FUSION ABSOLUTE is the first ultra-precise photon counting instrument enabling you to achieve absolute quantification. Its automated analysis capabilities allow you to detect, decode and quantify your protein of interest with certainty. This high-end system dedicated to all your chemiluminescence and fluorescence applications combines unrivalled optical sensitivity and optimum visualisation of your results to offer you the greatest level of performance. With its wide choice of customizable light sources, it’s a one-of-a-kind molecular imager opening the way to infinite multispectral imaging possibilities. Discover a world of extreme precision and technical innovations where your experiments know no limits.

BREAK THE BOUNDARIES OF PROTEIN DETECTION

FUSION ABSOLUTE custom made f/0.70 lens combines sensitivity and optical performance for very faint light conditions (picogram level). The system achieves the best signal to noise ratio and can easily detect large intensity difference between bright and faint bands before reaching saturation. The broad linear dynamic range enables quantification of target proteins with absolute confidence.

CHOOSE THE BEST OPTICS

The lens aperture represents its capability to collect as much light as possible in a given period. Its sensitivity is usually expressed by a range of f-stops. The smaller the f-stop number, the larger the aperture. A lower f-number denotes a greater aperture opening, which allows more light to reach the CCD sensor. The f/0.70 aperture of our lens is providing faster imaging and better sensitivity compared to all other imagers in the market. Our system is also equipped with a deeply cooled CCD scientific camera which allows you to get rid of background noise.

DISCOVER THE POWER OF MULTISPECTRAL IMAGING

Multispectral imaging in molecular imaging involves capturing images at multiple wavelengths to extract comprehensive information from a sample. It enhances the accuracy, versatility, and efficiency of the imaging process, making it a valuable tool for your research.

OBTAIN IMMEDIATE RESULTS

Vilber pushed the boundaries of detection, and significantly reduce acquisition time so that you can work faster and eventually save precious antibodies for chemiluminescence. For fluorescence, ultrasensitive detection capability facilitates the use of shorter excitation exposure. The FUSION ABSOLUTE protocol driven image acquisition is as quick as it is intuitive: adjust your exposure, save, print and quantify.

ALL YOU NEED IN ONE SYSTEM

Genomics: DNA analysis (mutation, methylation etc…), RNA (gene expression…) | Proteomics: Analysis of proteins molecular weight (analysis of mutation, expression, etc.) | Western blot: Biomedical research, molecular biology research, cancer research etc.

MULTIPLEXING

Multiplexing makes it possible to detect several different proteins in a single sample. This
technique will save you time and money, while giving you a complete analysis of the target protein.

REAGENTS & FLUOROPHORES

Fluorophores: Alexa 488 – Cy 2 – Alexa 532 – Alexa 555 – Alexa 680 – Alexa 700 – Alexa 750 – Cy 7- Alexa 790. Intercalating agent: Ethidium Bromide, SYBR Safe, Midori Green, Gel red, SYBR Green