ADAM MC2 - a new standard of Automated Cell Counter – Witec AG

Principle of Viability Measurement (PI-Staining Method)

After the samples are stained with ˜Fluorescent dye, propidium iodide, which intercalates DNA to stain the nucleus of target cells, ADAM takes ˜Fluorescent images automatically. The obtained images are processed by image analysis software integrated inside the system.

Counting Aggregated and Irregular-Shaped Cells

ADAM provides accurate and reliable results because it counts aggregated and irregular-shaped cells.

• Accurate count based on cell size and shape
• Count aggregated cells individually
• Debris is excluded from results

Accuracy & Repeatability

Correlation of total cell counting between hemocytometer and ADAM using SH-SY5Y cells.

Sample with low, medium and high concentration of cells were counted with ADAM. The repeatability at each level of cell concentration is high.

Comparison of Cell Viability

Comparison of cell viability between ADAM, flow cytometry, and manual counting.
SH-SY5Y, Jurkat, HeLa cells were treated with 100, 300μM H²O² for 3 hours, then analyzed by ADAM, flow cytometry, and manual counting.

Cell Therapeutic Applications

ADAM can be used as a device for monitoring and QC of the cell numbers and viability in the process of manufacturing cells (CAR-T cells, stem cells, etc.) for Cell Therapy.
In addition, it is possible to use ADAM depending on the cell types (Whole blood cell, PBMCs, etc.) that needs to be monitored during the manufacturing of cell therapy products.

• Stem cell
• CAR-T cell
• CAR-NK cell
• Adipose-derived stem cell
• Whole blood cell
• Aggregated cell
• PBMCs

QC Platform for Producing CAR-T cell

It is easy to monitor all different steps of the purification, expansion, and formulation of CAR-T cells using the ADAM to ensure precise and reliable results. ADAM can be used for cGMP, process control and quality control of CAR-T cell.

Performance Test from Isolated T Cells

The profiles of T cells which was used for performance evaluation – Flow cytometric analysis of CD3 expression on unsorted (WBC; left panel) or sorted (Isolated T cell; right panel) human peripheral blood lymphocytes.

Accuracy

Correlation of T cell counting between flow cytometry  and ADAM in Isolated T cells.

Method comparison between ADAM and flow cytometry.

Linearity

Comparison between flow cytometry and ADAM in Isolated T cells.

Repeatability

The sample that low, medium, and high concentration of isolated T cells were counted with ADAM.

Performance Test from Activated T Cells

The phenotypes of activated T cells which was used for performance evaluation – Flow cytometric analysis of CD25 expression on TCR stimulated (Activated T cell; bottom panel) or unstimulated (control T cell; upper panel) T cells.

Viability

Comparison of viability between flow cytometry and ADAM-CellT in Activated T cells.

Repeatability

The sample that low, medium, and high concentration of activated T cells were counted with ADAM.