UltraScript™ cDNA Synthesis Kit

Art No | PB30.15

UltraScript™ cDNA Synthesis Kit - Separate Oligos

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from 150.00 CHF

High-Performance cDNA Synthesis

Description

The UltraScript® cDNA Synthesis Kit uses the latest developments in reverse transcriptase technology and buffer chemistry to enhance cDNA synthesis speed, yield and representation. The reverse transcriptase, buffer system and combination of random hexamers with anchored oligo(dT) allow for unbiased, efficient and sensitive cDNA synthesis.

Unbiased representation of 5’ and 3’ mRNA ends
Sensitive detection of low copy number transcripts
High cDNA yields from as little as 4 pg total RNA
Simple 2 tube system
5x buffer contains anchored oligo(dT), random hexamers, enhancers, dNTPs and MgCl2
20x thermostable reverse transcriptase blended with RNase inhibitor
Also available in a format with separate oligos for maximum setup flexibility

UltraScript 2.0 cDNA Synthesis Kit Separate Oligos provides anchored oligo(dT) and random hexamers in separate tubes, for user optimisation depending on the type of analysis needed.

Applications

cDNA synthesis for real-time PCR analysis
Low copy number transcripts
Viral RNA targets
Efficient synthesis from total RNA or poly(A)+ RNA

Select the right product for your application

Primers	RNA Source	Intended Use	Recommended Product
Oligo(dT) & Random hexamers	Any	qPCR, cloning, NGS	PB30.11/PB30.15
Random hexamers	Prokaryotic, Archaeal	qPCR, cloning, NGS	PB30.15, (PB30.12 user must supply primer)
Oligo(dT)	Eukaryotic	qPCR, cloning, NGS	PB30.15, (PB30.12 user must supply primer)
Gene specific primer	Any	Cloning, targeted qPCR, targeted NGS	PB30.12 (user must supply primer)

Material Safety Data Sheet (PDF)

Product manual (PDF)

FAQs

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Supplier

PCR Biosystems
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Specifications

Reaction Volume	20μL
Storage	On arrival, products should be stored between -30 and -20 °C. If stored correctly the kit will retain full activity until the indicated expiry date.