UltraScript 2.0 cDNA Synthesis Kit

High-quality cDNA, even from challenging RNA

Description

The UltraScript 2.0 cDNA Synthesis Kit is engineered to deliver exceptional sensitivity and robustness, ensuring high-quality cDNA synthesis from a wide range of RNA samples. The proprietary UltraScript 2.0 reverse transcriptase is a highly thermostable and processive enzyme that works efficiently even with low-abundance or degraded RNA, as well as those with high GC content.

This kit is ideal for demanding applications, including real-time PCR (qPCR) and gene expression analysis.

Highly thermostable reverse transcriptase 55°C to 65°C and above
Advanced RNase inhibitor
High cDNA yields from as little as 20pg total RNA
Accurate reverse transcription of GC-rich and highly structured transcripts
High sensitivity for challenging templates
Reduced RNase H activity
Available as a stand-alone enzyme with buffer, a cDNA synthesis kit with premixed oligos and a cDNA synthesis kit with separate oligos

Applications

cDNA synthesis for PCR analysis, cloning, cDNA library preparation and Next Generation Sequencing
Viral RNA targets
miRNA targets
Efficient synthesis from total RNA or poly(A)+ RNA

The 2-tube UltraScript® 2.0 cDNA Synthesis Kit contains all the components required for fast, reliable and unbiased cDNA synthesis, including an optimised blend of anchored oligo(dT) and random hexamers. UltraScript® 2.0 cDNA Synthesis Kit Separate Oligos provides anchored oligo(dT) and random hexamers in separate tubes, for user optimisation depending on the type of analysis needed.

Material Safety Data Sheet (PDF)

Product manual (PDF)

FAQs

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Supplier

PCR Biosystems
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Specifications

Reaction Volume	20μL
Storage	On arrival, products should be stored between -30 and -20 °C. If stored correctly the kit will retain full activity until the indicated expiry date.