FAQs PCRBIO Ladders I-IV – Witec AG

Are PCRBIO Ladders stable at room temperature?

The ladders are already suspended in sample buffer and are ready for gel loading. The buffer formulation also allows for greater room temperature stability compared to conventional DNA ladders. Our ladders match or exceed stability of the best on the market.

What are the recommended applications for PCRBIO Ladders?

PCRBIO Ladders are designed for size determination and DNA quantification using agarose electrophoresis.

What are the sizes of PCRBIO ladders?

PCRBIO Ladder I (PB40.11): 100 – 10,000 bp

PCRBIO Ladder II (PB40.12): 250 – 10,000 bp

PCRBIO Ladder III (PB40.13): 50 – 1,500 bp

PCRBIO Ladder IV (PB40.14): 100 – 1,500 bp

Why do PCRBIO Ladders appear diffused on E-Gel Precast Agarose Electrophoresis System and how can I fix this?

The precast agarose systems are convenient however, there is a degree of compromise on the resolution. Any ladders will appear at much lower resolution on these systems. This is mainly due to the limited control they allow over the voltage as well as the gels themselves. A partial fix is to load less and ensure the ladder runs as far as it can. If the quality is still insufficient, we recommend using more traditional tanks and freshly cast gels.