Afyon SDS-PAGE Sample Preperation Kit – Witec AG

Kit for fast, easy concentration and purification of samples for SDS-PAGE

• Purify - Remove contaminants that can interfere with electrophoresis (GuHCl, urea, Ammonium sulfate, etc)
• Quick - Protein samples ready to load in less than 10 minutes
• Safe - and non-toxic - no DMSO required
• Compatible with SDS-PAGE and downstream Western blotting
• Many times faster than concentration and buffer exchange via ultrafiltration or dialysis

Concentrate and purify protein samples for SDS-PAGE with the Afyon sample purification kit.

Afyon SDS-PAGE sample preparation kit is a fast, efficient way to concentrate protein samples and remove buffer components that may interfere with electrophoresis. In less than 10 minutes, samples are ready for SDS-PAGE or immunoblotting. The Afyon protocol is easily scaled up and can be used for routine preparation of samples for electrophoresis and Western blotting.

Ready for electrophoresis in less than 10 minutes.

Afyon is a quick, easy protocol to concentrate protein samples and remove unwanted chemicals prior to electrophoresis. Simply add Afyon resin to the sample, vortex, spin to pellet resin, resuspend resin in gel loading buffer to elute protein, and remove resin with the spin filter provided with the Afyon kit. The resulting sample is ready to load on a gel in less than 10 minutes.