AdvanStain Ponceau (sufficient for staining 50 blots 9x7cm)

Art No | R-03021-D50

AdvanStain Ponceau (sufficient for staining 50 blots 9x7cm)

100.00 CHF

Brochure

Size: 500ml

Quick protein stain for membranes

Description

AdvanStain Ponceau rapidly detects proteins on nitrocellulose and PVDF membranes, allowing you to check the quality of protein transfer before proceeding to Western blotting.

With AdvanStain Ponceau, you can quickly make sure that protein transfer has been even across the entire blot, and that no signs of bubbles or other transfer artifacts are present. The staining is reversible, and after destaining membranes can immediately be used for Western blotting.

Quick - detect proteins in 5 minutes or less
Reversible - destain in minutes
Compatible - after destaining, PVDF and nitrocellulose membranes are ready for Western blotting

AdvanStain Ponceau (sufficient for staining 50 blots 9x7cm)

AdvanStain Ponceau was used to check protein transfer before Western blotting to detect solubilization of a protein of interest from inclusion bodies. Duplicate gels were run with the same set of protein samples and (a) one gel was stained with Commassie blue.

The other gel was transfered to a PVDF membrane, and (b) transferred protein was detected by staining the membrane with AdvanStain Ponceau. (c) After destaining with water, the membrane in (b) was subjected to Western blotting to detect the 72-KDa protein of interest. Lanes 1-3, solubilized inclusion bodies. Lanes 4-6, insoluble pellets.

The Western blot was detected using WesternBright Quantum HRP substrate.