Pre-cut WesternBright PVDF-FL transfer membranes - pre-cut & roll – Witec AG

Art No | Westernbright-PVDF-FL-membranes
from 117.00 CHF

PVDF-FL transfer membranes for Western transfer with fluorescent detection.

Description

Advansta offers a large selection of nitrocellulose and polyvinylidene difluoride (PVDF) transfer membranes for immunoblotting applications. All membranes are optimized for compatibility with a wide range of protein stains and enzyme-based detection systems, specifically with chemiluminescent substrates.

The WesternBright PVDF membranes provide durability and high tensile strength for ease of handling. The PVDF-FL membranes are perfect for fluorescent detection.

  • Pore Size: 0.22μm
  • Thickness: 135μm
  • Binding Interaction: Hydrophobic
  • Typical Protein Binding Capacity: 300 μg/cm2
  • Detection Methods: Fluorescence & total protein staining
  • Immobilization Methods: Electrophoretic transfer of proteins
  • Recommended Applications: Western transfer with fluorescent detection
  • Availables as: pre-cut membranes: 7x9cm, 10x15cm, 13x18cm or roll 26x330cm (0.45 μm)

Which Membrane is right for you?

Nitrocellulose

  • Traditional
  • Less expensive
  • Binding capacity: 80-100ug/cm2
  • Easily wetted prior to setting up the transfer
  • Lower background
  • Easy to block
  • Use with chicken antibodies
  • Comes in two pore sizes: 0.45um for proteins >20kDa & 0.2um for smaller proteins
  • Very fragile
  • Autofluorescent – cannot be used with fluorescent Westerns
  • Not compatible with SDS

PVDF

  • Binding capacity: 150 to 160ug/cm2
  • More expensive than nitrocellulose
  • Very resilient to mechanical and chemical damage
  • Compatible with fluorescent Westerns
  • Compatible with SDS-containing buffers
  • Requires activation step by wetting in methanol

Supplier

Advansta Inc.
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