WesternBright NC transfer membranes 0.22μm - pre-cut & roll

Art No | Westernbright-NC-membranes-22um

WesternBright NC transfer membranes 0.22μm - pre-cut & roll

Choose size / variation:

from 140.00 CHF

The WesternBright nitrocellulose membranes are pure nitrocellulose media that has high binding capacity for proteins.

Description

Advansta offers a large selection of nitrocellulose and polyvinylidene difluoride (PVDF) transfer membranes for immunoblotting applications. All membranes are optimized for compatibility with a wide range of protein stains and enzyme-based detection systems, specifically with chemiluminescent substrates.

The WesternBright nitrocellulose membranes are pure nitrocellulose media that has high binding capacity for proteins. These nitrocellulose membranes are stronger and more durable than other nitrocellulose membranes, and will not rip, tear, or crack during transfers. The nature of this material yields sharper images with little or no distortion.

Pore Size: 0.22μm
Thickness: 150μm
Binding Interaction: Hydrophobic, electrostatic
Typical Protein Binding Capacity: 200 μg/cm2
Detection Methods: Chemiluminescence, direct staining, radiolabeling & colorimetric
Immobilization Methods: Electrophoretic transfer of proteins, protein dot & slot blotting
Recommended Applications: Western transfer with chemiluminescent detection, Colony/plaque lifts
Availables as: pre-cut membranes: 6x8.5cm, 8x10cm or roll 30x300cm

Which Membrane is right for you?

Nitrocellulose

Traditional
Less expensive
Binding capacity: 80-100ug/cm²
Easily wetted prior to setting up the transfer
Lower background
Easy to block
Use with chicken antibodies
Comes in two pore sizes: 0.45um for proteins >20kDa & 0.2um for smaller proteins
Very fragile
Autofluorescent – cannot be used with fluorescent Westerns
Not compatible with SDS