FlashBlot-SD Semi-Dry Transfer Buffer - 500mL – Witec AG

Art No | R-03104-D50
200.00 CHF
200.00 CHF
Brochure

Size: 500mL

Rapid high efficiency semi-dry transfer buffer

Description

FLASHBlot™-SD Transfer Buffer is designed for rapid semi-dry transfer of proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using rapid semi-dry transfer systems. Transfer is compatible with commonly used detection methods such as membrane staining, chemiluminescent and fluorescent Western blotting.

  • FAST – high ionic strength formulation allows for protein transfer in 3 to 10-minutes when used with a compatible high current semi-dry blotting system
  • COMPATIBLE – use your existing high current semi-dry transfer apparatus
  • REPRODUCIBLE – consistent transfer across entire blot
  • VERSATILE – use nitrocellulose or PVDF membranes to achieve transfers with low background and high sensitivity with both chemiluminescent and fluorescent Western blots

FLASHBlot-SD produces chemiluminescent Western blots with highest sensitivity.

Chemiluminescent Western blot analysis of hnRNP K was performed on blots containing serially diluted HeLa lysate that was electrophoresed by SDS-Page then transferred to nitrocellulose membranes. Proteins were transferred from gel to membrane for 7 minutes at a constant current of 1.3 Amps.

FLASHBlot-SD transfer buffer

FLASHBlot-SD produces fluorescent Western blots with low background and high sensitivity.

IR fluorescent Western blot analysis of phosphorylated STAT3 and GAPDH was performed on blots containing serially diluted HeLa lysate (±IFNα treatment) that was electrophoresed by SDS-PAGE then transferred to PVDF membranes. Proteins were transferred from gel to membrane for 7 minutes at a constant current of 1.3 Amps.

FLASHBlot-SD Transfer Buffer

FLASHBlot-SD outperforms competitive semi-dry transfer buffers.

(a) AdvanStain Total-PVDF fluorescent protein membrane staining was performed on blots containing serially diluted HeLa lysate that was electrophoresed by SDS-PAGE then transferred to PVDF membranes. Proteins were transferred from gel to membrane for 7 minutes at a constant current of 1.3 Amps. (b) After membrane staining was complete, an IR800 fluorescent Western blot analysis of GAPDH was performed. FLASHBlot-SD produced 4-8 fold higher Western blot sensitivity in comparison to other commercially available transfer buffers.

FLASHBlot-SD Transfer Buffer

Supplier

Advansta Inc.
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