The Whole Picture. Whole Transcriptome, Hundreds of Proteins, Single Cell.
Description
Unbiased spatial profiling of the whole transcriptome and hundreds of protein markers on the same tissue section at single-cell resolution.
Combined with Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-seq), STOmics Stereo-CITE Proteo-Transcriptomics Set is intended for simultaneous detection of the whole transcriptome and 100+ plex protein on the same tissue section.
Stereo-CITE facilitates the study of tissue heterogeneity and associated biological significance in complex (disease) tissues, leading to a broader understanding of spatial omics studies.
- Spatial in situ multi-omics
Unbiased spatial profiling of the whole transcriptome and hundreds of protein markers on the same tissue section. - High Resolution
Subcellular resolution for both transcriptome and protein analysis. - High Specificity
Same section correlation analysis showed a high correlation between spatial protein distribution and protein fluorescence signals from immunofluorescence staining. - Reliable
Consistency analysis of three adjacent sections demonstrated good consistency between transcriptome and protein distributions. - Robust bioinformatic analysis pipeline
Deliver spatial maps of transcriptome and protein expression via sequencing and visualization.
Application Directions
- Organ heterogeneity studies
- Tumor microenvironment research
- Target discovery and drug development
- Immunotherapy
Validated Tissue Types
HUMAN TISSUE TYPES:
- Lung cancer
- Tonsil
- Thymus
- Esophagus
- Lymph node
- Cervical cancer
- Lymphoma
- Esophageal cancer
- Conlon cancer
- Renal cancer
- Gastric cancer
MOUSE TISSUE TYPES:
- Eshophageal cancer
- Spleen
- Liver
Technology Principle
Utilizing the ultra-high resolution of Stereo-seq, Stereo-CITE combines Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-seq) with the Stereo-seq workflow. It captures mRNA and antibody-derived tags (ADTs) at nanoscale resolution across a centimeter-scale field of view. Through a streamlined “tissue-to-data” workflow, the system delivers spatial maps of transcriptome and protein expression via sequencing and visualization.
Specifications
| 211SP11118 |
Stereo-seq Permeabilization Set for Chip-on-a-slide V1.1
For determining permeabilization parameters to optimize mRNA capture. |
| 211PT11114 |
Stereo-CITE Proteo-Transcriptomics Set V1.1
For generating a spatially-resolved 3’ mRNA library and ADT (protein) library from the same biological tissue sections. |
| 212KA11114 |
Stereo-seq Protein Assisted Kit
This accessory kit provides additional reagents required for proteo-transcriptomic workflows and is included in the Stereo-CITE |
| 111KL160 |
Stereo-seq 16 Barcode Library Preparation Kit
Designed for library preparation of samples using Stereo-seq technology, enables the addition of sample barcodes and library construction. |
| 301AUX001 |
Stereo-seq PCR Adaptor
Compatible with PCR thermal cycler as a heating unit. |