STOmics Stereo-CITE V1.1 Proteo-Transcriptomics Solution – Witec AG

Art No | STEREO-CITE
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The Whole Picture. Whole Transcriptome, Hundreds of Proteins, Single Cell.

Description

Unbiased spatial profiling of the whole transcriptome and hundreds of protein markers on the same tissue section at single-cell resolution.

Combined with Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-seq), STOmics Stereo-CITE Proteo-Transcriptomics Set is intended for simultaneous detection of the whole transcriptome and 100+ plex protein on the same tissue section.
Stereo-CITE facilitates the study of tissue heterogeneity and associated biological significance in complex (disease) tissues, leading to a broader understanding of spatial omics studies.

  • Spatial in situ multi-omics
    Unbiased spatial profiling of the whole transcriptome and hundreds of protein markers on the same tissue section.
  • High Resolution
    Subcellular resolution for both transcriptome and protein analysis.
  • High Specificity
    Same section correlation analysis showed a high correlation between spatial protein distribution and protein fluorescence signals from immunofluorescence staining.
  • Reliable
    Consistency analysis of three adjacent sections demonstrated good consistency between transcriptome and protein distributions.
  • Robust bioinformatic analysis pipeline
    Deliver spatial maps of transcriptome and protein expression via sequencing and visualization.

Application Directions

  • Organ heterogeneity studies
  • Tumor microenvironment research
  • Target discovery and drug development
  • Immunotherapy

Validated Tissue Types

HUMAN TISSUE TYPES:

  • Lung cancer
  • Tonsil
  • Thymus
  • Esophagus
  • Lymph node
  • Cervical cancer
  • Lymphoma
  • Esophageal cancer
  • Conlon cancer
  • Renal cancer
  • Gastric cancer

MOUSE TISSUE TYPES:

  • Eshophageal cancer
  • Spleen
  • Liver

Technology Principle

Utilizing the ultra-high resolution of Stereo-seq, Stereo-CITE combines Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-seq) with the Stereo-seq workflow. It captures mRNA and antibody-derived tags (ADTs) at nanoscale resolution across a centimeter-scale field of view. Through a streamlined “tissue-to-data” workflow, the system delivers spatial maps of transcriptome and protein expression via sequencing and visualization.

Supplier

MGI Tech Latvia
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Specifications

211SP11118

Stereo-seq Permeabilization Set for Chip-on-a-slide V1.1

  • 8 RNX
  • V1.1

For determining permeabilization parameters to optimize mRNA capture.

211PT11114

Stereo-CITE Proteo-Transcriptomics Set V1.1

  • 4 RNX
  • V1.1

For generating a spatially-resolved 3’ mRNA library and ADT (protein) library from the same biological tissue sections.

212KA11114

Stereo-seq Protein Assisted Kit

  • 4 RNX
  • V1.1

This accessory kit provides additional reagents required for proteo-transcriptomic workflows and is included in the Stereo-CITE
Proteo-Transcriptomics Set V1.1. Can be ordered separately.

111KL160

Stereo-seq 16 Barcode Library Preparation Kit

  • 16 RNX
  • V1.0

Designed for library preparation of samples using Stereo-seq technology, enables the addition of sample barcodes and library construction.

301AUX001

Stereo-seq PCR Adaptor

  • 2 EA

Compatible with PCR thermal cycler as a heating unit.

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