NGSBIO DNA Standards for Illumina® | 500 x 20μL Reactions

Art No | PB71.22-05

NGSBIO DNA Standards for Illumina® | 500 x 20μL Reactions

330.00 CHF

Brochure

Size: 500 x 20μL Reactions

The DNA standards supplied are precisely measured and ready-to-use.

Description

Wide Dynamic Range

The DNA standards supplied are precisely measured and ready-to-use, covering 5 orders of magnitude from 2pM to 0.2fM. The kit is suitable for quantification of even low concentration libraries including libraries constructed without a PCR amplification step.

Compatible with all Illumina® instruments and qPCR platforms

Material Safety Data Sheet (PDF)

Product manual (PDF)

FAQs

Figure 1. Amplification Curves

An adapter-ligated library sample (purple) is run alongside six standard templates (grey) provided in the NGSBIO Library Quant Kit.

Figure 2. Standard Curve

The Cts of the amplification curves are plotted against the log of the concentration of the standard templates. A linear curve is fitted through the standards. The concentration of the unknown sample is then calculated from its position on the curve.

Figure 3. High consistency and reproducibility of quantification

Quantification of 16 adapter-ligated libraries using NGSBIO Library Quant Kit (purple), NEBNext® Library Quant Kit
(orange) and KAPA Library Quantification Kit (green).
The NGSBIO Library Quant Kit shows less spread and greater consistency among replicates. The quantification results are within those obtained by two leading manufacturers of NGS library quantification kits. The number on the bottom right corner of each graph represents the concentration of dsDNA obtained using a Qubit Fluorometer from Invitrogen.