IsoFast™ Bst 1-Step Mix

Art No | PB80.21

IsoFast™ Bst 1-Step Mix

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from 320.00 CHF

IsoFast™ Bst 1-Step Mix is a dual enzyme system for rapid and sensitive isothermal amplification of RNA targets in one step.

Description

The kit contains IsoFast™ Bst Polymerase, providing strong strand displacement capabilities, together with the highly active modified MMLV RTase Go.

Validated for qualitative detection of SARS-CoV-2 nucleic acid
Includes strand-displacing IsoFast™ Bst Polymerase in a 2x mix format
Supplied with RTase Go plus RNase inhibitor
Reaction is carried out at 65˚C
Gives rapid and consistent amplification across a wide range of templates
Includes an advanced buffer system for higher yield under difficult conditions
Supplied with a fluorescent dye for real-time detection
30 minute protocol

IsoFast™ Bst 1-Step Mix is designed for reverse transcription of target RNA and subsequent isothermal amplification in a single tube.

Dual enzyme system

The kit utilises IsoFast™ Bst Polymerase for its strong strand displacement activity, enabling DNA synthesis at a constant temperature (65˚C) without the need for thermal cycling. Representing the large fragment of Geobacillus stearothermophilus (formerly Bacillus stearothermophilus) DNA Polymerase, this portion of the protein catalyses the 5’-3’ synthesis of DNA but does not contain the 5’-3’ exonuclease domain.¹The enzyme is provided in a convenient 2x mix format.

Reverse transcription of target RNA is carried out by the thermostable and extremely active RTase Go, which is blended with RNase inhibitor to prevent degradation of RNA by contaminating RNase.

Rapid and consistent results

Designed for fast amplification, IsoFast™ Bst 1-Step Mix gives rapid and consistent results across a wide range of sample types and is validated for qualitative detection of SARS-CoV-2 nucleic acid. The buffer chemistry ensures high yield and performance even under difficult conditions, for example when inhibitors are present.

IsoFast™ Bst 1-Step Mix contains all the components for fast and sensitive isothermal amplification of RNA targets, requiring only the addition of primers, template and water. The kit is provided with a separate tube of fluorescent dye to allow real-time detection with any qPCR instrument.

Applications

Multiple displacement amplification
Isothermal amplification
Reverse Transcription Loop mediated isothermal amplification (RT-LAMP)
Molecular diagnostics
Field diagnostics

This product is not suitable for PCR.

¹Mead DA, McClary JA, Luckey JA, Kostichka AJ, Witney FR, Smith LM. Bst DNA polymerase permits rapid sequence analysis from nanogram amounts of template. Biotechniques. 1991 Jul;11(1):76-8, 80, 82-87.

Product manual (PDF)

FAQs

Isothermal Amplification

Isothermal amplification refers to a range of methods that rely on an alternative approach to amplification that is not limited by the requirement for thermal cycling.

Instead of denaturing DNA by using high temperatures, isothermal amplification takes advantage of DNA polymerases that have high strand displacement capabilities, for example Bst or Phi29 DNA polymerases. These enzymes have the ability to ‘unzip’ DNA as they synthesise complementary strands, removing the need for thermocycler equipment as the reaction is carried out at a one temperature. Isothermal methods making use of strand displacing DNA polymerases include multiple displacement amplification, whole genome amplification and loop mediated isothermal amplification or LAMP.

PCR Biosystems’ IsoFast™ Bst range of products is based on the large fragment of Bst DNA polymerase and offers rapid and sensitive amplification of DNA and RNA targets at a constant temperature of around 65°C.

Supplier

PCR Biosystems
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Specifications

IsoFast Bst Mix	100 Reactions: 1 x 1.25mL 500 Reactions. 4 x 1.6mL
RTase Go	100 Reactions: 1 x 200µL 500 Reactions: 1 x 1mL
20x Fluorescent Dye	100 Reactions: 1 x 125µL 500 Reactions: 1 x 625µL
Reaction Volume	25µL