Art No | PB10.43
VeriFi™ Mix - Long range and high fidelity PCR
from 170.00 CHF
PCRBIO VeriFi™ Mix Red is a convenient high fidelity 2x mix designed for PCR applications where greater sequence accuracy is required, together with improved PCR success rates of long and challenging templates. The inclusion of a red dye enables direct loading and tracking during agarose gel electrophoresis.
Description
PCRBIO VeriFi™ Mix Red contains the engineered and highly processive PCRBIO VeriFi™ Polymerase, developed for fast and versatile high fidelity PCR. The enzyme is derived from Pfu DNA polymerase for its 3’-5’ exonuclease (proofreading) activity. Several proprietary mutations significantly improve DNA binding and processivity, resulting in shorter extension times (10-30s/kb), higher yields and the ability to amplify longer and more difficult targets, including eukaryotic genomic templates in excess of 17.5kb.
- High temperature cycling – up to 100°C denaturation to better separate GC-rich templates
- Increased PCR success rates with complex genomic templates (17.5kb and over)
- High yields under standard and fast PCR conditions (10-30s/kb)
- Efficient and specific amplification from challenging templates including GC and AT-rich sequences
- 100x higher fidelity than Taq DNA polymerase
- Generates blunt-end PCR products
The high accuracy and enhanced 3’-5’ exonuclease activity of PCRBIO VeriFi™ Polymerase result in fidelity that is approximately 100 times higher than Taq DNA polymerase. The enzyme is ideally suited to applications where greater accuracy is required, such as cloning, site-directed mutagenesis and sequencing. PCR products generated with this range of products are blunt ended.
PCRBIO VeriFi™ Mix Red uses an advanced buffer system including dNTPs, Mg and enhancers, enabling high fidelity PCR of a wide range of targets and fragment sizes with minimal or no optimisation required.
Applications
- High fidelity PCR
- Long range PCR
- Site-directed mutagenesis
- Cloning
- Sequencing
Storage
On arrival, products should be stored between -30 and -15°C. If stored correctly the kit will retain full activity for 12 months.
Figure 1. Versatility across a broad range of amplicon lengths
PCRBIO VeriFi™ Polymerase amplifies the range of fragment lengths indicated with high yield and specificity. The starting template amount is 4-30ng of mouse or human genomic DNA, diluted 1.5 to 3 fold. GC content ranges from 37-55%. L: PCRBIO Ladder II.
Figure 2. Increased success rates with complex templates
Amplification of a 17.5kb fragment of the HBB gene. The starting template amount is 150ng (A and C) and 30ng (B and D) of human genomic DNA, diluted 2 fold. A 2-step PCR protocol was used with amplification at 72°C (A and B) or 68°C (C and D). GC content is 37%. PCRBIO VeriFi™ Polymerase amplifies long fragments with yields comparable to Takara PrimeSTAR GXL DNA Polymerase. L: PCRBIO Ladder II.
Figure 3. Amplification of targets with high sensitivity and specificity compared to leading competitors
Amplification of a 1.0kb fragment of the GAPDH gene with different starting template amounts of mouse genomic DNA. A: 20ng, B: 3.2ng, C: 0.5ng, D: 0.08ng. GC content is 51%. L: PCRBIO Ladder IV. The reactions were set up following manufacturers’ recommendations. Cycling conditions were 95°C 2 min, then 30 cycles of 98°C 15 sec, 66°C 15 sec and 72°C 30 sec. PCRBIO VeriFi™ Polymerase displays greater sensitivity and specificity compared to leading competitors.
Available sizes:
| PB10.44-01 | 2 x 1.25mL | 100 Reactions |
| PB10.44-05 | 10 x 1.25mL | 500 Reactions |
Specifications
| 2x PCRBIO VeriFi™ Mix Red |
2 x 1.25mL | 100 Reactions 10 x 1.25mL | 500 Reactions |
| Reaction Volume |
50μL |
Related products
Art No | PB10.42
VeriFi™ Polymerase - Long range and high fidelity PCR (Incl. dNTPs)
from 110.00 CHF