Size: 5mL
GenScript Streptavidin Resin is an affinity chromatography medium designed for easy, one-step purification of biotinylated peptides, antibodies, lectins, etc from samples.
Description
The purified recombinant streptavidin is covalently coupling to 4% highly cross-linked agarose. The coupling is optimized to give high binding capacity for biotinylated molecules. The total binding capacity of Streptavidin Resin is more than 120 nmol of D-Biotin/ml settled resin.
- High biotin-binding capacity, more than 120 nmol of D-biotin per ml of column-volume, much higher than that of competitors
- RNase, DNase, and protease free
- Competitive price
Streptavidin is a biotin-binding protein found in the culture broth of the bacterium Streptomyces avidinii . Streptavidin binds 4 moles of biotin per mole of protein with an extremely high affinity. Streptavidin lacks carbohydrate side chains present on avidin and has an isoelectric point of 6.5 (vs 10 for avidin) close to where most useful biological interactions occur. As a result, streptavidin frequently exhibits much lower non-specific binding than avidin does.
Note: High temperature heating is not recommended. The agarose melts above 65°C.