High Affinity Ni-Charged Resin | 50% slurry – Witec AG

GenScript High Affinity Ni-Charged Resin is an 4% cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins.

High Affinity Ni-Charged Resin has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin the excellent choice for high performance purification of polyhistidine-tagged proteins.

• High binding capacity: The kit can handle over 20 mg 6xHis-tagged protein/ml (CV).
• Simple purification procedure under both native and denaturing conditions.
• Broad pH stability: The resin works well between pH 3 and pH 13, and can manage pH 2 and pH 14 for short periods.
• Resilient: If properly regenerated, the resin can be reused multiple times.

Applications

• Purification of polyhistidine-tagged proteins under native conditions
• Purification of polyhistidine-tagged proteins from E. coli under denaturing conditions