Size: 1mL
GenScript Anti-DYKDDDDK IP Resin is designed for quick and efficient immunoprecipitation of DYKDDDDK-tagged protein or DYKDDDDK-tagged protein-target protein complex.
Description
The resin is an agarose beads coupled to Mouse anti-DYKDDDDK monoclonal antibody (clone 6H8E4). The antibody recognizes N-terminal, internal and C-terminal fusion protein containing DYKDDDDK tag.
It is supplied as a suspension in 50 % glycerol containing 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.4,0.02 % (w/v) sodium azide (PBS/A).
- High DYKDDDDK-tag binding capacity
- Low levels of nonspecific binding
- Used for immunoprecipitation of DYKDDDDK-tagged protein or DYKDDDDK-tagged protein-target protein complex
Comparison between Anti-DYKDDDDK IP Resin and Anti-DYKDDDDK G1 Affinity Resin.
Anti-DYKDDDDK G1 Affinity Resin (L00432) | Anti-DYKDDDDK IP Resin (L00425) | ||
---|---|---|---|
Immunoprecipitation (IP) | √ | √ | |
Purification | √ | n/a | |
Elution | Alkaline elution | √ | n/a |
Alkalescent elution | √ | n/a | |
Acid elution | √ | n/a | |
Neutral elution | √ | n/a | |
Competitive elution | √ | n/a | |
SDS-PAGE loading synthesize buffer | √ | √ | |
Regeneration | 4-10 times | not recommend | |
Binding volume | large scale | mini-volume (<1ml) |