DYKDDDDK tag is an octapeptide tag that can be added to a protein using recombinant DNA technology.
Description
It can be fused to the N-terminus or C-terminus of a target protein to facilitate detection and purification.
GenScript Anti-DYKDDDDK G1 Affinity Resin is designed for the purification of DYKDDDDK-tagged protein from commonly used protein expression systems including bacteria, yeast and mammalian cells. The DYKDDDDK-tagged protein in the cell lysate can specifically bind to the anti-DYKDDDDK monoclonal antibodies coupled to the resin. Nonspecific bindings are eliminated by stringent washing steps, and the target protein is finally eluted with high recovery rate.
- High DYKDDDDK binding capacity and ultralow levels of non-specific binding
- Multiple elution methods for diverse protein characteristics and different requirements
- Used for purification and immunoprecipitation of DYKDDDDK-tagged protein or protein complex
Comparison between Anti-DYKDDDDK IP Resin and Anti-DYKDDDDK G1 Affinity Resin.
Anti-DYKDDDDK G1 Affinity Resin (L00432) | Anti-DYKDDDDK IP Resin (L00425) | ||
---|---|---|---|
Immunoprecipitation (IP) | √ | √ | |
Purification | √ | n/a | |
Elution | Alkaline elution | √ | n/a |
Alkalescent elution | √ | n/a | |
Acid elution | √ | n/a | |
Neutral elution | √ | n/a | |
Competitive elution | √ | n/a | |
SDS-PAGE loading synthesize buffer | √ | √ | |
Regeneration | 4-10 times | not recommend | |
Binding volume | large scale | mini-volume (<1ml) |
Specifications
Product content |
50% settled resin in TBS with 0.02% sodium azide |
Matrix |
4% cross-linked agarose |
Average bead size |
90 μm |
Ligand |
Mouse monoclonal antibody against DYKDDDDK tag, clone G1 |
Binding capacity |
Approximately 1 mg DYKDDDDK-tagged protein per ml settled resin |
Storage and stability |
Store at 2-8 °C for up to 12 months. Do not freeze the resin. |
Resin reuse |
The resin can be recycled for at least 4 times. If maintained properly, the resin can be reused 10 times with minimum loss of binding capability |
Elution method |
Acid, alkaline, neutral, peptide competitive or PAGE loading buffer NOTE: Elution with SDS-PAGE loading buffer causes denaturation of the G1 antibody. |
Reagents compatibility |
Compatible with commonly used reagents at certain concentrations |