High Affinity Ni-Charged Resin FF

Art No | L00666

High Affinity Ni-Charged Resin FF

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from 87.00 CHF

GenScript High Affinity Ni-Charged Resin FF is an 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins.

Description

High Affinity Ni-Charged Resin FF has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification.

This makes High Affinity Ni-Charged Resin FF the excellent choice for high performance purification of polyhistidine-tagged proteins.

Binding capacity: >40 mg of histidine-tagged protein /mL settled resin
Average particle size: 90 μm (45-165 μm)

Compatible reagents:

Denaturants	Detergents	Reducing agents	Salts	Others
6 M Gu·HCl	2% Triton X-100	20 mM β-ME	4 M MgCl₂	50% glycerol
8 M Urea	2% Tween 20	1 mM DTT	5 mM CaCl₂	20% ethanol
	1% CHAPS		2 M NaCl	1 mM EDTA

Volumes supplied in 50% slurry

Selection Guide Resins (PDF)

Supplier

Genscript
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Specifications

Binding capacity	>40 mg of histidine-tagged protein /mL settled resin
Matrix spherical	6% highly cross-linked Agarose
Average particle size	90 μm (45-165 μm)
Storage solution	20% Ethanol
Storage	Store at 2-8°C – do NOT freeze