Immobilization Buffer Optimization Kit – Witec AG

Immobilization buffers are used to dilute the ligand for direct coupling onto the sensor surface of Carboxyl-functional sensors.

Immobilization Buffer Optimization Kit contains a panel of 10 mM Sodium Acetate buffers from pH 4.0-5.5.

Immobilization buffers are used to dilute the ligand for direct coupling onto the sensor surface of Carboxyl-functional sensors.

The low pH and salt content is ideal for pre-concentration of the ligand to the Carboxyl sensor surface, increasing immobilization efficiency. It is recommended to find the optimal pH for a ligand experimentally. 10 mM Sodium Acetate Buffers are ideal for many applications where the ligand pI is at or below 6.

Kit contains:

• 1x 10 mM Acetate Buffer, pH 4.0, 20 mL
• 1x 10 mM Acetate Buffer, pH 4.5, 20 mL
• 1x 10 mM Acetate Buffer, pH 5.0, 20 mL
• 1x 10 mM Acetate Buffer, pH 5.5, 20 mL