Running Buffer HBS-EP pH 7.4 – Witec AG

Hepes Buffered Saline (HBS) is a running buffer used throughout a typical interaction experiment on OpenSPR instruments.

Tween 20 is a common additive to help reduce non-specific binding and sample loss, and EDTA helps to chelate any heavy metal ions present in the buffer solution.

Provided at 1x concentration, HBS-EP contains 0.01 M HEPES, 0.15 M NaCl, 0.005% Tween 20, 3 mM EDTA at pH 7.4.

Reagent is provided at a volume of 400 mL and comes ready-to-use.