AmMag™ SA semi-automated Protein/Antibody purification System – Witec AG

Protein purification in under an hour instead of days with AmMag™  - a magnetic bead-based, high-quality and affordable protein and antibody purification platform.

GenScript's AmMag™ SA automates the protein or antibody purification using magnetic beads. The instrument is capable of using 6-well deep well culture plates or 50 mL tubes. In combination with antibody or recombinant protein binding magnetic beads the system offers a high throughput with a high capacity.

• Purifies up to 12 samples at a time
• Sample volumes from 5mL to 50mL
• Up to 80mg of protein or antibody per sample
• Typical sample time for 12 samples is less than 40 minutes

The instrument features an integrated touchscreen and is controlled via its user-friendly software interface. The closed environment minimizes the chance of endotoxin contamination during purification.

Eliminate the need for centrifugation and filtration

The AmMag™ SA utilizes magnetic beads for purification. Since magnetic beads are free-flowing, cells and debris from cultures do not prevent them from binding target protein and antibodies. This eliminates the need to clear cultures by centrifugation and filtration prior to binding with magnetic beads.

Complete washing and elution process automatically

After cultures have expressed the desired proteins or antibodies, magnetic beads are added to bind the targets. After this step, the cultures are transferred to 50 mL tubes and then moved to AmMag™ SA. The automated washing and elution steps purify the target proteins and antibodies from the magnetic beads.

Closed environment to minimize endotoxin contamination

The AmMag™ SA is equipped with air filters to clean incoming air, and further maintains a positive air flow through the instrument. These features prevent airborne endotoxins from entering the system and contaminating samples. To clean the instrument between runs, all tubing can be flushed and incubated for up to an hour with 0.1 N NaOH.

Use with larger samples (few litres)

The AmMag™ SA contains adapters for entering samples in 15 mL and 50 mL tubes. However, larger samples can be easily purified using the AmMag™ SA by simply collecting the magnetic beads from these samples after the binding step, and transferring the beads into 50 ml or 15 ml tubes. To help collect magnetic beads from larger samples, we provide the AmMag™ Wand and the AmMag™ Block.