qPCRBIO SyGreen Blue Mixes

Art No | PB20.15-17

qPCRBIO SyGreen Blue Mixes

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from 59.00 CHF

See the mix, skip the mistakes.

Description

qPCRBIO SyGreen® Mix combines a proprietary non-inhibiting intercalating dye with the latest advances in polymerase technology and buffer chemistry to give you fast, highly sensitive and reproducible dye-based real-time qPCR.

qPCRBIO SyGreen® Mix can be used to reliably quantify any DNA template including genomic, cDNA and viral sequences using dye-based qPCR, and is able to detect extremely low copy number targets with the highest efficiency.

Capable of ultra-fast cycling (down to 2 s denaturation, 2 s annealing/extension)
Rapid extension rate for early Ct values
Market-leading sensitivity – increased limit of detection
Specific amplification from complex templates including GC-rich sequences
Non-PCR inhibiting intercalating dye
Also available as an easy-to-see blue mix
Compatible with all real-time instruments

SyGreen Blue Mixes from PCR Biosystems offer a high-performance, blue-dyed master mix for qPCR. The visible dye prevents pipetting errors, ensuring accuracy and reliability for sensitive DNA analysis.

The 2x mix uses a proprietary DNA intercalating dye, that does not inhibit PCR, unlike other popular fluorescent dyes. The same developments that improve the sensitivity and consistency of qPCRBIO SyGreen® Mix in standard cycling conditions also allow for industry-leading performance in fast and ultra fast cycling conditions.

qPCRBIO SyGreen® Blue Mix contains a non-reactive dye to improve reaction mix visibility and enable sample visualisation in reaction plate wells, allowing greater pipetting precision and reduced errors without affecting your real-time PCR performance.

Applications

Relative gene expression analysis
Absolute quantification
High-throughput qPCR from genomic, cDNA and viral sequences
Detection of extremely low copy number targets
Crude sample PCR

SYBR Green vs SyGreen, do they perform the same way?

Yes, SyGreen and SYBR Green (registered trademark of Molecular Probes Inc. and is owned by Thermo Fischer Scientific) use the same molecular mechanism to generate signal in qPCR and SyGreen reagents are an alternative to SYBR Green-containing master mixes.

How does qPCRBIO SyGreen® work?

These intercalating dyes possess aromatic and planar groups that allow the intercalation of the molecule in the minor groove of double-stranded DNA spanning approximately 3.5-4 nucleotides. The positive groups present stabilise the dyes allowing for the coordination with the negatively charged DNA backbone.

Due to this mechanism of binding to DNA, they are not able to bind to single-strand nucleic acids, unless secondary structures are formed. On the other hand, fluorescence increases up to 1,000-fold upon intercalation with dsDNA.

When a dye intercalates between DNA base pairs, it emits fluorescence proportional to the amount of bound dye (and hence to the amount of DNA in a sample). This allows visualisation of nucleic acids on gels or fluorimeters in real time qPCR instruments. In the latter case, the number of intercalated dye molecules in a sample increases with the progress of a qPCR, leading to a corresponding increase in fluorescence with each cycle. At the onset of the reaction, the free dye emits a basal fluorescence level. As the reaction proceeds the fluorescence intensity increases exponentially, before it plateaus in later cycles, leading to the classic sigmoidal amplification plot.

Instrument Compatibility Tool

FAQs

PCRBIO Tm Calculator

Amplification of Beta-2 Microglobulin using qPCRBIO SyGreen Mix (purple curves).

Amplification curves are shown in the top panel of set a and set b, melt curves are shown in the middle panel and the efficiencies of amplification are shown in the bottom panel.

A direct, on-plate comparison was performed with the competitors identified in the top panel of each set (grey curves). 5 serial dilutions of mouse cDNA template were used in a total reaction volume of 10µL. Cycling conditions were those recommended by each of the competitors.

qPCRBIO SyGreen Mix displays earlier Ct, cleaner melt peaks and better efficiency compared to each of the competitor mixes.

Supplier

PCR Biosystems
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Specifications

Reaction Volume	20μL
Instrument Compatibility	This product is compatible with all standard and fast cycling qPCR instruments. Use our qPCR Selection Tool to find out which ROX variant is compatible with your instrument.
ROX Concentrations	qPCRBIO SyGreen Mix Lo-ROX contains 112 nM ROX. qPCRBIO SyGreen Mix Hi-ROX contains 1.12 µM ROX. qPCRBIO SyGreen Mix Separate-ROX 2x mix contains no ROX and include a separate tube of 50 µM ROX additive. This enables you to choose what concentration of ROX you’d like to use.
Storage	On arrival, products should be stored between -30 and -20 °C. If stored correctly the kit will retain full activity until the indicated expiry date.