Art No | PB20.11-14
qPCRBIO SyGreen Mixes
from 59.00 CHF
One Step. Superior Performance.
Description
qPCRBIO SyGreen® 1-Step Kits have been designed for fast, highly specific and ultra-sensitive cDNA synthesis and real-time PCR in a single tube.
Select from qPCRBIO SyGreen® 1-Step Detect for the detection of extremely low copy number targets, or qPCRBIO SyGreen® 1-Step Go for rapid and accurate results from high RNA template concentrations.
- Thermostable reverse transcriptase 45 °C to 55 °C
- Advanced RNase inhibitor
- Non-PCR inhibiting intercalating dye
- Rapid extension rate for early Ct values
- Market-leading sensitivity
- Increased limit of detection
- Antibody-mediated hot start PCR
- Compatible on all standard and fast cycling real-time PCR platforms
qPCRBIO SyGreen 1-step Detect has been engineered to have high sensitivity for rapid and accurate results from low template concentrations 1pg of total RNA or 0.01pg of mRNA.
The kits include a thermostable and extremely active modified MMLV reverse transcriptase and advanced RNase inhibitor that prevents degradation of RNA by contaminating RNase. PCR amplification is powered by antibody-mediated hot start technology, preventing the formation of primer dimers and non-specific products leading to improved reaction sensitivity and specificity. Combining the latest developments in polymerase technology and advanced buffer chemistry we offer market- leading performance with minimal or no optimisation.
Applications
- Relative gene expression analysis
- Absolute quantification
- Detection of extremely low copy number targets
Specifications
| Reaction Volume |
20μL |
| Instrument Compatibility |
This product is compatible with all standard and fast cycling qPCR instruments. Use our qPCR Selection Tool to find out which ROX variant is compatible with your instrument. |
| Storage |
On arrival, products should be stored between -30 and -20 °C. If stored correctly the kit will retain full activity until the indicated expiry date. |
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