qPCRBIO HRM Mix

Art No | PB20.31

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from 53.00 CHF

qPCRBIO HRM Mix - developed for reliable and accurate detection of genetic mutations and SNPs using High Resolution Melting (HRM) analysis.

Description

qPCRBIO HRM Mix produces ultra-sensitive melt profiles allowing accurate discrimination of subtle sequence variations including class I to class IV mutations and CpG methylation differences.

Fast and accurate detection of subtle sequence differences
Ultra-sensitive fluorescence profiles for clear characterisation of samples
Third generation saturating dye SyGreen 2
Antibody-mediated hot start technology for improved sensitivity
Compatible with all real-time instruments with HRM capability

High Resolution Melt (HRM) is a powerful technique used immediately following PCR for the analysis of mutations, polymorphisms and epigenetic differences in double stranded DNA samples. Samples are characterised based on DNA strand dissociation behavior as temperature is increased in the presence of a fluorescent dye. HRM analysis exploits the differences in melt curve shapes and DNA melting temperature to discriminate sequence variations between samples.

qPCRBIO HRM Mix uses SyGreen 2, a third generation non-PCR inhibiting saturating dye which produces ultra-sensitive melt profiles capable of discriminating class I to IV mutations and CpG methylation differences. Unlike first generation dyes, SyGreen 2 can be used at high concentrations and does not have a different affinity for AT or GC rich sequences.

This 2x mix uses antibody-mediated hot start technology to prevent the formation of primer dimers and non-specific products, leading to improved reaction sensitivity and specificity. Combining the latest advancements in polymerase technology and advanced buffer chemistry we offer fast, reliable and cost effective HRM analysis with minimal or no optimisation required.

SNP genotyping
Gene scanning
CpG methylation analysis

Use our qPCR Selection Tool to find out which ROX variant is compatible with your instrument.

Storage

The kit should be stored between -30°C and -15°C. Avoid prolonged exposure to light. If stored correctly the kit will retain full activity for 12 months. The kit can be stored at 4°C for 1 month. The kit can go through 30 freeze/thaw cycles with no loss of activity.

Material Safety Data Sheet (PDF)

Product manual (PDF)

Instrument Compatibility Tool

FAQs

Figure 1. Class IV SNP

Three samples of human genomic DNA were amplified using qPCRBIO HRM Mix with primers specific for a fragment of beta-globulin gene. The primers flanked a class IV single nucleotide polymorphism (17A/T). After amplification the products were subjected to HRM analysis.

The 3 traces show clear allele calling of a class IV SNP.

The black trace is for homozygous A, the purple trace homozygous T and the green trace heterozygous green.

Figure 2. Class I SNP

Three samples of human genomic DNA were amplified using qPCRBIO HRM Mix with primers specific for a fragment of Factor V gene. The primers flanked a class I single nucleotide polymorphism (1691G/A). After amplification the products were subjected to HRM analysis.

The 3 traces show clear allele calling of a class I SNP.

The black trace is for homozygous A, the purple trace homozygous G and the green trace heterozygous green.

Figure 3. Class II SNP

Three samples of human genomic DNA were amplified using qPCRBIO HRM Mix with primers specific for a fragment of MTHFR gene. The primers flanked a class II single nucleotide polymorphism (1298A/C). After amplification the products were subjected to HRM analysis.

The 3 traces show clear allele calling of a class II SNP.

The black trace is for homozygous A, the purple trace homozygous C and the green trace heterozygous green.

Figure 4. Class III SNP

Three samples of human genomic DNA were amplified using qPCRBIO HRM Mix with primers specific for a fragment of
HFE gene. The primers flanked a class III single nucleotide polymorphism (187C/G). After amplification the products were subjected to HRM analysis.

The 3 traces show clear allele calling of a class III SNP.

The blue trace is for homozygous C, the red trace homozygous G and the green trace heterozygous green.