Lyo-Ready Probe Kit

Lyophilisable RT-qPCR kit designed for ultra-sensitive detection of RNA sequences.

Description

These specially formulated mixes are ideal for the development of diagnostic assays.

They contains a glycerol-free 4x qPCR mix comprising hot start Taq polymerase, dNTPs, MgCl2 and a blend of excipients to provide optimal functionality without loss of activity post lyophilisation.

Ultra-sensitive detection of RNA sequences
Offers the same high performance post-lyophilisation as our standard kits
Includes a glycerol-free 4x qPCR mix
Pre-blended with excipients for reliable lyophilisation into beads or cakes
Provided with separate, high-concentration UltraScript® RTase (with RNase inhibitor)
Validated for qualitative detection of SARS-CoV-2 nucleic acid
Compatible with high-throughput, highly-multiplexed assays
4x qPCR mix also available separately for DNA detection

Applications

Development of lyophilised molecular diagnostic tests
Detection of extremely low copy number targets
Compatible with TaqMan®, Scorpions® and molecular beacon probes

Optimised for lyophilisation

Lyophilisation offers many advantages including ambient storage, extended shelf-life and increased versatility in sample volume. The kit has been optimised to offer the same high performance post-lyophilisation as our standard 1-step RT-qPCR kits.

Robust and reliable RT-qPCR

Lyo-Ready Probe 1-Step Kit includes a glycerol-free 4x qPCR mix containing hot start Taq polymerase, dNTPs, MgCl₂ and a blend of excipients to ensure reliable lyophilisation, without loss of activity. Antibody-mediated hot start technology ensures specific amplification of virus-derived cDNA, with improved tolerance to the common PCR inhibitors found in clinical samples.

UltraScript® Reverse Transcriptase is provided separately in a highly concentrated 3200x format. This robust and thermostable modified MMLV reverse transcriptase is engineered to enhance cDNA synthesis speed and yield with accurate transcript representation and enables fast and efficient reverse transcription up to 55 °C.

Material Safety Data Sheet (PDF)

Product manual (PDF)

Catalogue

PCRBIO Tm Calculator