Air-Dryable Probe 1-Step Mixes

Versatile all-in-one, airdryable 4x qPCR and RT-qPCR reagent mixes for ultra-sensitive detection of DNA and RNA sequences.

Description

Air-Dryable Probe Mix and Probe 1-Step Mix are versatile all-in-one, air-dryable 4x qPCR and RT-qPCR reagent mixes for ultra-sensitive detection of RNA and DNA sequences.

Designed for rapid air-drying, these qPCR and RT-qPCR mixes enable dried assay formats using a standard laboratory oven, offering a simpler, faster, and cheaper alternative to lyophilisation. An optimal blend of excipients ensures reliable air-drying, without loss of activity. Choose Air-Dryable Probe Mix when working with DNA only, or use Air-Dryable Probe 1-Step Mix for efficient detection of both DNA and RNA targets.

Unbiased, sensitive detection of both DNA and RNA targets, 4 copies per reaction
Complete 1-step RT-qPCR mix, single tube format
Easy reaction set up, add only primers & probes before drying
Rapid air-drying protocol, 80-90 min drying time
Same high performance before & after drying
Room temperature storage and shipping of dried reactions
Increased sample volume input

Applications

Diagnostic probe-based qPCR & RT-qPCR
DNA & RNA detection
TaqMan®, Scorpions® and molecular beacon probe qPCR
Multiplex and singleplex qPCR setup

The Air-Dryable Probe 1-Step Mix includes a glycerol-free 4x qPCR mix containing hot start Taq polymerase, dNTPs, MgCl₂ and a blend of excipients to ensure reliable air-drying, without loss of activity. The mastermix also contains a novel version of UltraScript Reverse Transcriptase, modified to increase stability upon drying, and our RiboShield™ RNase inhibitor, so the final blend is suitable both for qPCR detection of DNA and 1-Step RT-qPCR for detection of RNA targets.

Designed for rapid air-drying, Air-Dryable Probe 1-Step Mix is intended for use in in-vitro diagnostic kit development (IVD) but can also be used for anyone wishing to benefit from the advantages of dried (RT-)qPCR reactions. Air-drying protocols require significantly less time and expertise than freeze drying (lyophilisation) and very simple equipment. 5 μL of 4x Air-Dryable Probe 1-Step Mix (required for a 20 μL final reaction volume) can be dried down into a gel using a standard laboratory oven in as little as 80-90 min.

Air-Dryable Probe 1-Step Mix is presented in a single tube format in order to make assay setup for 1-step probe-based RT-qPCR detection of RNA or DNA as simple as possible. The mix is specially formulated for air-drying of the reaction mix, requiring only the addition of primers and probes prior to drying. Reconstitution of the mix is equally simple, necessitating addition only of sample and, optionally, water to make up the final reaction volume. A quick vortexing of the plate will guarantee full reconstitution of the gel.

Key benefits of air-drying

Reduced volume and mass of reaction mixes leads to cheaper shipping and storage.
Room temperature stability of the air-dried final gel eliminates the need for cold-chain shipping and storage. This simplifies logistics and reduces overall cost.
Increased volumes of sample can be used. The sample input volume can equal the final reaction volume. This is perfect for dilute samples (with a low target copy number).
Air-drying in an oven is a highly accessible method, because there is no requirement for specialist equipment and knowledge, eliminating the need for additional staff or project out-sourcing
Increased control over the drying process and final product development for small and medium sized IVD kit developers

Material Safety Data Sheet (PDF)

Product manual (PDF)

Catalogue

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