Art No | PB20.61-64
Clara® Probe Mix
from 170.00 CHF
Easy-to-see purple dye
Description
Clara® Probe Purple Mix offers reliable probe-based qPCR detection of DNA target sequences. Combined with a non-inhibitory purple dye for easy sample visualisation, this 4x mix offers superior target amplification, in single or multiplex assays, even from highly dilute samples.
Clara® Probe Purple Mix offers the same reliable, sensitive and specific performance of our latest probe-based qPCR mixes. Additionally, the mix is supplemented with a unique purple dye for sample visualisation during plate setup. This dye is non-inhibitory to PCR and doesn’t affect commonly used fluorescent probes, with minimal quenching, which ensures strong signal generation for accurate measurements.
- Concentrated 4x mix format, ideal for high-throughput, highly multiplexed assays
- Superior detection of DNA targets
- Reliable quantification of low template amounts
- Reduced primer dimer formation for high specificity
- Antibody-mediated hot start technology
- Compatible with all real-time PCR platforms – standard and fast cycling conditions
- With inert purple dye for easy visualisation during setup
- For RNA detection, use Clara® Probe 1-Step Mix.
Powerful qPCR mix
Clara® Probe Purple Mix is engineered to enable qPCRs with the highest sensitivity and reliability, and with the greatest ease of use in basic research and diagnostic applications alike. It is a universal 4x qPCR mix suitable for use with all types of probe technologies, including TaqMan, Scorpions and molecular beacons.
Powered by PCR Biosystem’s unique hot start Taq DNA polymerase, Clara® Probe Purple Mix is suitable for DNA detection in 2-step RT-qPCR protocols when used with a separate cDNA synthesis kit.
Clara® Probe Purple Mix can be used for all types of probe-based qPCR applications, including gene expression analysis, SNP/allele detection, genotyping and allelic discrimination studies, and species abundance quantification.
Applications
- 2-step RT-qPCR
- Species abundance quantification
- Genotyping
- Allelic discrimination
- In vitro diagnostic kit development
- Single & multiplex DNA detection
AquaPlex passive reference dye
The AquaPlex dye has a fluorescence emission spectrum suitable for red (Cy5) channel detection, allowing use of the ROX channel forprobe dyes. This leads to improved data quality in multiplex reactions, because probes using the Cy5 dye often result in poorer quality signal compared to other channels. Additionally, unlike ROX, there is no requirement for different dye concentrations with various real-time PCR instruments.
Specifications
| Reaction Volume |
20μL |
| Instrument Compatibility |
This product is compatible with all standard and fast cycling qPCR instruments. Use our qPCR Selection Tool to find out which ROX variant is compatible with your instrument. |
| Storage |
On arrival, products should be stored between -30 and -20 °C. If stored correctly the kit will retain full activity until the indicated expiry date. |