Art No | PB20.65-68
Clara® Probe Purple Mix
from 170.00 CHF
Better chemistry, better results.
Description
Clara® Probe Mix is the newest solution for qPCRs. With clear and consistent results, this cutting-edge qPCR mix will streamline your real-time PCR workload no matter what the application.
Clara® Probe Mix is a next-generation qPCR mastermix designed to give you reliable results with superior sensitivity. This optimised 4x formulation is engineered for maximum reliability in both single and multiplex assays. It is suitable for diagnostic test development and basic research and is universally compatible with all probe types and qPCR instruments. For crude saliva or blood sample input and broad-spectrum inhibitor-tolerance, please try our Clara® Inhibitor-Tolerant Probe Mix.
- Concentrated 4x mix format, ideal for high-throughput, highly multiplexed assays
- Superior detection of DNA targets
- Reliable quantification of low template amounts
- Reduced primer dimer formation for high specificity
- Antibody-mediated hot start technology
- Compatible with all real-time PCR platforms – standard and fast cycling conditions
- Available with inert purple dye for easy visualisation during setup
- For RNA detection, use Clara® Probe 1-Step Mix.
Reliable Probe qPCR
Clara® Probe Mix is engineered to enable qPCRs with the highest sensitivity, reliability, and with the greatest ease of use in diagnostic applications and basic research alike. It is a universal qPCR mix suitable for all types of probe technologies, including TaqMan, Scorpions and molecular beacons. Powered by our unique hot start Taq DNA polymerase the qPCR mix is suitable for DNA detection for 2-step RT-qPCR protocols when used with a separate cDNA synthesis kit. Although for routine RNA quantification we recommend 1-step protocols with Clara® Probe 1-Step Mix for a more streamlined workflow.
Clara® Probe Mix can be used for all types of probe-based qPCR applications, including gene expression analysis, SNP/allele detection, genotyping and allelic discrimination studies, species abundance quantification, and offers high efficiency in both single and multiplex assays. Making this mix highly versatile and ideal for in-vitro diagnostic kit development as well as basic research.
Applications
- 2-step RT-qPCR
- Species abundance quantification
- Genotyping
- Allelic discrimination
- In vitro diagnostic kit development
- Single & multiplex DNA detection
AquaPlex passive reference dye
The AquaPlex dye has a fluorescence emission spectrum suitable for red (Cy5) channel detection, allowing use of the ROX channel forprobe dyes. This leads to improved data quality in multiplex reactions, because probes using the Cy5 dye often result in poorer quality signal compared to other channels. Additionally, unlike ROX, there is no requirement for different dye concentrations with various real-time PCR instruments.
Specifications
| Reaction Volume |
20μL |
| Instrument Compatibility |
This product is compatible with all standard and fast cycling qPCR instruments. Use our qPCR Selection Tool to find out which ROX variant is compatible with your instrument. |
| Storage |
On arrival, products should be stored between -30 and -20 °C. If stored correctly the kit will retain full activity until the indicated expiry date. |