Clara™ Probe Mix Lo-ROX – Witec AG

Clara™ Probe Mix is the newest solution for qPCRs. With clear and consistent results, this cutting-edge qPCR mix will streamline your real-time PCR workload no matter what the application.

Clara™ Probe Mix is a next-generation qPCR mastermix designed to give you reliable results with superior sensitivity. This optimised 4x formulation is engineered for maximum reliability in both single and multiplex assays. It is suitable for diagnostic test development and basic research and is universally compatible with all probe types and qPCR instruments.

• Concentrated 4x mix format, ideal for high-throughput, highly multiplexed assays
• Superior detection of DNA targets
• Reliable quantification of low template amounts
• Reduced primer dimer formation for high specificity
• Antibody-mediated hot start technology
• Compatible with all real-time PCR platforms – standard and fast cycling conditions

Reliable Probe qPCR

Clara™ Probe Mix is engineered to enable qPCRs with the highest sensitivity, reliability, and with the greatest ease of use in diagnostic applications and basic research alike. It is a universal qPCR mix suitable for all types of probe technologies, including TaqMan®, Scorpions® and molecular beacons. Powered by our unique hot start Taq DNA polymerase the qPCR mix is suitable for DNA detection for 2-step RT-qPCR protocols when used with a separate cDNA synthesis kit. Although for routine RNA quantification we recommend 1-step protocols with Clara™ Probe 1-Step Mix for a more streamlined workflow.

Clara™ Probe Mix can be used for all types of probe-based qPCR applications, including gene expression analysis, SNP/allele detection, genotyping and allelic discrimination studies, species abundance quantification, and can offers high efficiency in both single and multiplex assays. Making this mix highly versatile and ideal for in-vitro diagnostic kit development as well as basic research.

The mix is also suitable for melt-curve analysis (with hybridisation probes only) and is available without passive reference dye (No-Rox), or with reverence dye as Lo-ROX, Hi-Rox and separate-ROX formulations.

Use our qPCR Selection Tool to find our which ROX variant is compatible with your instrument.