Clara® Probe 1-Step Purple Mix

Art No | PB25.85-88

Clara® Probe 1-Step Purple Mix

Size:

Passive reference:

from 320.00 CHF

Brochure Select variation

Precise, fast, and simple one-step qPCR.

Description

Clara® Probe 1-Step Purple Mix ensure RT-qPCRs are no longer a challenge. This new single tube RT-qPCR mix is suitable for universal probe-based detection of RNA and DNA targets in single and multiplex assays.

This carefully balanced 4x RT-qPCR mix combines maximum sensitivity with ease-of-use for streamlined RT-qPCR workflows. As a result, the mix shows no loss of efficiency in amplifying DNA or RNA and is ideal for users who detect both types of targets without the requirement for separate qPCR reagents. It is also Available in both clear and purple formats for easy mastermix visualisation. For crude saliva or blood sample input and broad-spectrum inhibitor-tolerance, please try the Clara® Inhibitor-Tolerant Probe 1-Step Mix.

Concentrated 4x mix format, ideal for high-throughput, highly multiplexed assays
Ultra-sensitive detection of RNA & DNA targets
Early detection of a wide range of template amounts
Contains modified UltraScript® RTase in a single-tube format
Advanced RNase inhibitor
Antibody-mediated hot start technology
Compatible with all real-time PCR platforms – standard and fast cycling conditions
Available with inert purple dye for easy visualisation during setup

Probe 1-step qPCR mix – Clear Results, Reliable Conclusions

Clara® Probe 1-Step Mix enables qPCR and 1-step RT-qPCR with the highest sensitivity, reliability, and with the greatest ease of use in diagnostic applications and basic research alike. It is a universal qPCR mix suitable for all types of probe technologies, including TaqMan, Scorpions and molecular beacons. This is our first generation of all-in-one 1-step RT-qPCR mixes, where both the DNA polymerase and reverse transcriptase are included in the same mastermix. Clara® Probe 1-Step Mix is powered by our unique hot start Taq DNA polymerase and a specially modified version of our high performance, thermostable UltraScript® Reverse Transcriptase. Rigorous testing during development makes this mix highly suitable for both single and multiplex detection with high efficiency and sensitive detection whether you’re detecting one target or many simultaneously.

This mix is also suitable for melt-curve analysis (with hybridisation probes only) and is available without passive reference dye (No-Rox), or with reverence dye as Lo-ROX, Hi-Rox, Separate-ROX, and AquaPlex formulations. Use our qPCR Selection Tool to find out which ROX variant is compatible with your instrument.

AquaPlex formats are designed with a passive reference dye suitable for detection in the red (Cy5, 650 nm) channel, enabling the use of probe dyes that can be monitored in the channel normally used for ROX detection. This allows for more optimal signal generation and better quality data in multiplex assays.

Applications