Clara™ Probe 1-Step Mix Separate-ROX

Art No | PB25.84

Clara™ Probe 1-Step Mix Separate-ROX

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from 320.00 CHF

RT-qPCRs are no longer a challenge. Get clear and consistent results every time with Clara™ Probe 1-Step Mix. This new single tube RT-qPCR mix is suitable for universal probe-based detection of RNA and DNA targets in single and multiplex assays.

Description

Easy-to-use, the single tube 4x RT-qPCR mix is suitable for universal probe-based detection of RNA and DNA targets in single and multiplex assays. With both DNA polymerase and reverse transcriptase in the same mastermix, the Probe 1-Step Mix makes qPCR as simple and streamlined as possible, allowing you to investigate RNA targets and conduct DNA quantification in the same run, while minimising the risk of contamination. The mix can detect down to four copies of target per 20 μL reaction.

This carefully balanced 4x RT-qPCR mix combines maximum sensitivity with ease-of-use for streamlined 1-step probe RT-qPCR workflows. As a result, the mix shows no loss of efficiency in amplifying DNA or RNA and is ideal for users who detect both types of targets without the requirement for separate qPCR reagents.

Concentrated 4x mix format, ideal for high-throughput, highly multiplexed assays
Ultra-sensitive detection of RNA & DNA targets
Early detection of a wide range of template amounts
Contains modified UltraScript™ RTase in a single-tube format
Advanced RNase inhibitor
Antibody-mediated hot start technology
Compatible with all real-time PCR platforms – standard and fast cycling conditions

Probe 1-step qPCR mix – Clear Results, Reliable Conclusions

Clara™ Probe 1-Step Mix enables qPCR and 1-step RT-qPCR with the highest sensitivity, reliability, and with the greatest ease of use in diagnostic applications and basic research alike. It is a universal qPCR mix suitable for all types of probe technologies, including TaqMan®, Scorpions® and molecular beacons. This is our first generation of all-in-one 1-step RT-qPCR mixes, where both the DNA polymerase and reverse transcriptase are included in the same mastermix. Clara™ Probe 1-Step Mix is powered by our unique hot start Taq DNA polymerase and a specially modified version of our high performance, thermostable UltraScript™ Reverse Transcriptase. Rigorous testing during development makes this mix highly suitable for both single and multiplex detection with high efficiency and sensitive detection whether you’re detecting one target or many simultaneously.

Get the greatest ease of use

Our proprietary buffer composition (identified via smart screen technology) and uniquely modified UltraScript™ RTase allow the mix to be stored in a single tube, as all-in-one, RT-qPCR mastermix. This reduces the amount of pipetting needed for assay setup, minimising the risk of contamination, and saving time. It also means that one reagent is suitable for amplification of DNA and RNA and both target types can be included on the same plate or in the same run. Having one mix means you do not require a different setup when primarily investigating RNA targets, but still requiring DNA quantification.

While users who test large quantities of both target types of targets may prefer dedicated reagents for each target type, Clara™ Probe Mix is developed exclusively for DNA and cDNA probe-based detection.

Improve reliability of real time PCR

Extensive optimization makes this mix suitable for all nucleic acid target types. We have tested it against common RNA viruses, including SARS-CoV-2, RSV, Influenza A, and B, standard housekeeping genes, such as g-actin and GAPDH, as well as DNA targets. The presence of RTase in the mix does not affect direct amplification of DNA targets, meaning you get the same high efficiency whether you’re detecting DNA or RNA sequences. The mix is highly stable and shows strong reproducibility between runs, giving your data reproducibility and confidence in the experimental conclusions you draw.

Maximise 1-step qPCR sensitivity

The high concentration 4x mix format of Clara™ Probe 1-Step Mix offers greater sensitivity and flexibility by allowing more sample to be added to each reaction, and for smaller reaction volumes to be used with confidence. Fast amplification allows for earlier Cts and permits reliable detection of down to four target copies per 20 μL reaction (0.8 copies per μL). Get the superior detection even in the most dilute samples.