Art No | PB25.85-88
Clara® Probe 1-Step Purple Mix
from 320.00 CHF
Precise, fast, and simple one-step qPCR.
Description
Clara® Probe 1-Step Mix ensure RT-qPCRs are no longer a challenge. This new single tube RT-qPCR mix is suitable for universal probe-based detection of RNA and DNA targets in single and multiplex assays.
This carefully balanced 4x RT-qPCR mix combines maximum sensitivity with ease-of-use for streamlined RT-qPCR workflows. As a result, the mix shows no loss of efficiency in amplifying DNA or RNA and is ideal for users who detect both types of targets without the requirement for separate qPCR reagents. It is also Available in both clear and purple formats for easy mastermix visualisation. For crude saliva or blood sample input and broad-spectrum inhibitor-tolerance, please try the Clara® Inhibitor-Tolerant Probe 1-Step Mix.
- Concentrated 4x mix format, ideal for high-throughput, highly multiplexed assays
- Ultra-sensitive detection of RNA & DNA targets
- Early detection of a wide range of template amounts
- Contains modified UltraScript® RTase in a single-tube format
- Advanced RNase inhibitor
- Antibody-mediated hot start technology
- Compatible with all real-time PCR platforms – standard and fast cycling conditions
- Available with inert purple dye for easy visualisation during setup
Probe 1-step qPCR mix – Clear Results, Reliable Conclusions
Clara® Probe 1-Step Mix enables qPCR and 1-step RT-qPCR with the highest sensitivity, reliability, and with the greatest ease of use in diagnostic applications and basic research alike. It is a universal qPCR mix suitable for all types of probe technologies, including TaqMan, Scorpions and molecular beacons. This is our first generation of all-in-one 1-step RT-qPCR mixes, where both the DNA polymerase and reverse transcriptase are included in the same mastermix. Clara® Probe 1-Step Mix is powered by our unique hot start Taq DNA polymerase and a specially modified version of our high performance, thermostable UltraScript® Reverse Transcriptase. Rigorous testing during development makes this mix highly suitable for both single and multiplex detection with high efficiency and sensitive detection whether you’re detecting one target or many simultaneously.
This mix is also suitable for melt-curve analysis (with hybridisation probes only) and is available without passive reference dye (No-Rox), or with reverence dye as Lo-ROX, Hi-Rox, Separate-ROX, and AquaPlex formulations. Use our qPCR Selection Tool to find out which ROX variant is compatible with your instrument.
AquaPlex formats are designed with a passive reference dye suitable for detection in the red (Cy5, 650 nm) channel, enabling the use of probe dyes that can be monitored in the channel normally used for ROX detection. This allows for more optimal signal generation and better quality data in multiplex assays.
Applications
- Gene expression analysis
- Genotyping
- Allelic discrimination
- In vitro diagnostic kit development
- Single & multiplex RNA & DNA detection
AquaPlex passive reference dye
The AquaPlex dye has a fluorescence emission spectrum suitable for red (Cy5) channel detection, allowing use of the ROX channel forprobe dyes. This leads to improved data quality in multiplex reactions, because probes using the Cy5 dye often result in poorer quality signal compared to other channels. Additionally, unlike ROX, there is no requirement for different dye concentrations with various real-time PCR instruments.
Specifications
| Reaction Volume |
20μL |
| Instrument Compatibility |
This product is compatible with all standard and fast cycling qPCR instruments. Use our qPCR Selection Tool to find out which ROX variant is compatible with your instrument. |
| Storage |
On arrival, products should be stored between -30 and -20 °C. If stored correctly the kit will retain full activity until the indicated expiry date. |