Clear results, even with inhibitors.
Description
Get cutting edge DNA detection even in the presence of PCR inhibitors with Clara® Inhibitor-Tolerant Probe Mix. This broad-spectrum, inhibitor-tolerant qPCR mix is expertly designed to overcome challenging laboratory, environmental, and clinical sample inhibitors, ensuring reliable results and streamlined workflows for DNA detection.
- Broad spectrum inhibitor tolerance
- Concentrated 4x mix format, ideal for high-throughput, highly multiplexed assays
- Superior detection of DNA targets
- Reliable quantification of low template amounts
- Antibody-mediated hot start technology
- Compatible with all real-time PCR platforms – standard and fast cycling conditions
- Standard and ultra-fast cycling
Powerful qPCR mix
Clara® Inhibitor-Tolerant Probe Mix is engineered to enable qPCRs with the highest sensitivity and reliability on the toughest of samples. It is a universal 4x qPCR mix suitable for use with all types of probe technologies, including TaqMan, Scorpions and molecular beacons. Powered by our unique hot start Taq DNA polymerase, Clara® Inhibitor-Tolerant Probe Mix is suitable for DNA detection and for RNA detection in 2-step RT-qPCR protocols when used with a separate cDNA synthesis kit.
Robust Inhibitor Tolerance
Clara® Inhibitor-Tolerant Probe 1-Step Mix has been extensively tested against:
- Crude saliva (10%)
- Crude blood (6%)
- Laboratory chemicals (SDS, guanidine, ethanol)
- Clinical inhibitors (hemin, hemoglobin, heparin, lactoferrin, immunoglobulins, urea)
- Plant, and environmental inhibitors (humic acid, catechin, quercetin, tannic acid, cellulose, and chlorophyll).
Applications
- Species abundance quantification
- Genotyping
- Allelic discrimination
- In vitro diagnostic kit development
- Single & multiplex DNA detection
- Crude saliva qPCR
Specifications
| Reaction Volume |
20μL |
| Instrument Compatibility |
This product is compatible with all standard and fast cycling qPCR instruments. Use our qPCR Selection Tool to find out which ROX variant is compatible with your instrument. |
| Storage |
On arrival, products should be stored between -30 and -20 °C. If stored correctly the kit will retain full activity until the indicated expiry date. |