Clara® Inhibitor-Tolerant Probe 1-Step Mix Lo-ROX

Art No | PB25.91

Clara® Inhibitor-Tolerant Probe 1-Step Mix Lo-ROX

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from 410.00 CHF

Broad spectrum inhibitor tolerance

Description

Clara® Inhibitor-Tolerant Probe 1-Step Mix offers broad-spectrum inhibitor-tolerant 4x mix for probe-based 1-step RT-qPCR.

Unlock the power of precise RT-qPCR analysis with Clara® Inhibitor-Tolerant Probe 1-Step Mix. This broad spectrum, inhibitor-tolerant RT-qPCR mix is expertly designed to overcome challenging laboratory, environmental, and clinical sample inhibitors, ensuring reliable results and streamlined workflows for your research. For end-users wishing to amplify DNA only we recommend Clara® Inhibitor-Tolerant Probe Mix.

Broad spectrum inhibitor tolerance
Concentrated 4x mix format, ideal for high-throughput, highly multiplexed assays
Single- and multiplex detection of DNA and RNA
Contains modified UltraScript® RTase in a single-tube format
Advanced RNase inhibitor
Antibody-mediated hot start technology
Compatible with all real-time PCR platforms – standard and fast cycling conditions
Standard and ultra-fast cycling (down to 3 min RT reaction and 1 s denaturation, 3 s annealing/extension for PCR)

Applications

In vitro diagnostic kit development
Single & multiplex RNA & DNA detection
Crude saliva qPCR & RT-qPCR
Gene expression analysis
Genotyping
Allelic discrimination

What is Clara® Inhibitor-Tolerant Probe 1-Step Mix?

PCR and qPCR can be inhibited by a wide variety of chemical compounds, negatively affecting qPCR workflows and diagnostic processes. Clara® Inhibitor-Tolerant Probe 1-Step Mix is a 4x ready-mix that includes a DNA polymerase, reverse transcriptase (RTase), RNase inhibitor, buffering agents, magnesium, dNTPs, and other necessary components for successful probe-based (RT-)qPCR, all in a single-tube format. The end user can combine this mix with primers, probes, and templates for single- or multiplex detection. This mix is universally compatible with all probe chemistries, including TaqMan, Scorpions, and molecular beacons, and is maximally compatible with all commercial real-time PCR instruments. Clara® Inhibitor-Tolerant Probe 1-Step Mix is designed for use in in vitro diagnostic (IVD) kit development and can also be used in various research applications requiring efficient, reliable (RT-)qPCR reactions.

What type of templates is this mix suitable for?

The 1-step mix can be used to amplify both DNA and RNA samples without a loss in efficiency, regardless of template type. Clara® Inhibitor-Tolerant Probe 1-Step Mix has been extensively engineered for use in crude saliva testing but tolerates a wide range of clinical and laboratory-derived qPCR inhibitors.

Types of inhibitors Clara® Inhibitor-Tolerant Probe 1-Step Mix tolerates.

The Clara® Inhibitor-Tolerant Probe 1-Step Mix has been tested against, and is tolerant to, a broad range of common PCR inhibitors, including standard laboratory chemicals (SDS, guanidine, and ethanol) and biological sample inhibitors, such as those found in blood samples (hemin, hematin, hemoglobin, heparin, IgG immunoglobulins, lactoferrin, sodium citrate), saliva, urine (urea), plant, and environmental samples (humic acid, catechin, quercetin, tannic acid, cellulose, and chlorophyll). The mix has been extensively validated against crude (or diluted) saliva and blood, along with multiple other pure chemical inhibitors.

Material Safety Data Sheet (PDF)

Product manual (PDF)

Catalogue