Reliable qPCR, even with inhibitors.
Description
Unlock the power of precise RT-qPCR analysis with Clara® Inhibitor-Tolerant Probe 1-Step Mix. This broad-spectrum, inhibitor-tolerant RT-qPCR mix is expertly designed to overcome challenging laboratory, environmental, and clinical sample inhibitors, ensuring reliable results and streamlined workflows for your research.
- Broad spectrum inhibitor tolerance
- Concentrated 4x mix format
- Single and multiplex detection of DNA and RNA
- Contains modified UltraScript® RTase in a single-tube format
- Advanced RNase inhibitor
- Antibody-mediated hot start technology
- Compatible with all real-time PCR platforms – standard and fast cycling conditions
- Standard and ultra-fast cycling
All-in-one qPCR mix
Clara® Inhibitor-Tolerant Probe 1-Step Mix is an all-in-one, 4x mastermix for 1-step RT-qPCR with universal probe compatibility. It contains RNase inhibitor along with a uniquely modified version of UltraScript® RTase so the mix can be stored in a single tube. This reduces the amount of pipetting needed for assay setup, minimising the risk of contamination and saving time.
Inhibitor-tolerant RT-qPCR mix
Clara® Inhibitor-Tolerant Probe 1-Step Mix has been extensively tested against:
- Crude saliva (10%)
- Crude blood (6%)
- Laboratory chemicals (SDS, guanidine, ethanol)
- Clinical inhibitors (hemin, hemoglobin, heparin, lactoferrin, immunoglobulins, urea)
- Plant, and environmental inhibitors (humic acid, catechin, quercetin, tannic acid, cellulose, and chlorophyll).
Applications
- Gene expression analysis
- Genotyping
- Allelic discrimination
- In vitro diagnostic kit development
- Single & multiplex RNA & DNA detection
- Crude saliva qPCR & RT-qPCR
Specifications
| Reaction Volume |
20μL |
| Instrument Compatibility |
This product is compatible with all standard and fast cycling qPCR instruments. Use our qPCR Selection Tool to find out which ROX variant is compatible with your instrument. |
| Storage |
On arrival, products should be stored between -30 and -20 °C. If stored correctly the kit will retain full activity until the indicated expiry date. |