Clara® Inhibitor-Tolerant Probe 1-Step Mix – Witec AG

Art No | PB25.91-94
from 420.00 CHF

Reliable qPCR, even with inhibitors.

Description

Unlock the power of precise RT-qPCR analysis with Clara® Inhibitor-Tolerant Probe 1-Step Mix. This broad-spectrum, inhibitor-tolerant RT-qPCR mix is expertly designed to overcome challenging laboratory, environmental, and clinical sample inhibitors, ensuring reliable results and streamlined workflows for your research.

  • Broad spectrum inhibitor tolerance
  • Concentrated 4x mix format
  • Single and multiplex detection of DNA and RNA
  • Contains modified UltraScript® RTase in a single-tube format
  • Advanced RNase inhibitor
  • Antibody-mediated hot start technology
  • Compatible with all real-time PCR platforms – standard and fast cycling conditions
  • Standard and ultra-fast cycling

All-in-one qPCR mix

Clara® Inhibitor-Tolerant Probe 1-Step Mix is an all-in-one, 4x mastermix for 1-step RT-qPCR with universal probe compatibility. It contains RNase inhibitor along with a uniquely modified version of UltraScript® RTase so the mix can be stored in a single tube. This reduces the amount of pipetting needed for assay setup, minimising the risk of contamination and saving time.

 

Inhibitor-tolerant RT-qPCR mix

Clara® Inhibitor-Tolerant Probe 1-Step Mix has been extensively tested against:

  • Crude saliva (10%)
  • Crude blood (6%)
  • Laboratory chemicals (SDS, guanidine, ethanol)
  • Clinical inhibitors (hemin, hemoglobin, heparin, lactoferrin, immunoglobulins, urea)
  • Plant, and environmental inhibitors (humic acid, catechin, quercetin, tannic acid, cellulose, and chlorophyll).

Applications

  • Gene expression analysis
  • Genotyping
  • Allelic discrimination
  • In vitro diagnostic kit development
  • Single & multiplex RNA & DNA detection
  • Crude saliva qPCR & RT-qPCR

Supplier

PCR Biosystems
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Specifications

Reaction Volume

20μL

Instrument Compatibility

This product is compatible with all standard and fast cycling qPCR instruments.

Use our qPCR Selection Tool to find out which ROX variant is compatible with your instrument.

Storage

On arrival, products should be stored between -30 and -20 °C. If stored correctly the kit will retain full activity until the indicated expiry date.

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