Art No | PB10.43
VeriFi™ Mix - Long range and high fidelity PCR
from 170.00 CHF
PCRBIO VeriFi™ Polymerase is a versatile and robust high fidelity enzyme engineered for all PCR applications where greater sequence accuracy is required.
Description
Improved DNA binding and increased processivity result in shorter extension times, higher yields and the ability to amplify longer and more difficult targets.
- High temperature cycling – up to 100°C denaturation to better separate GC-rich templates
- Increased PCR success rates with complex genomic templates (17.5kb and over)
- High yields under standard and fast PCR conditions (10-30s/kb)
- Efficient and specific amplification from challenging templates including GC and AT-rich sequences
- 100x higher fidelity than Taq DNA polymerase
- Generates blunt-end PCR products
- Available as a convenient 2x ready mix with the option of a red dye for direct gel loading
PCRBIO VeriFi™ Polymerase is derived from Pfu DNA polymerase for its 3’-5’ exonuclease (proofreading) activity. The enzyme is engineered with proprietary mutations that significantly increase processivity, resulting in shorter extension times (10-30 s/kb), higher yields and the ability to amplify longer and more difficult targets, including eukaryotic genomic templates in excess of 17.5kb.
The high accuracy and enhanced 3’-5’ exonuclease activity of PCRBIO VeriFi™ Polymerase result in fidelity that is approximately 100 times higher than Taq DNA polymerase. The enzyme is ideally suited to applications where greater accuracy is required, such as cloning, site-directed mutagenesis and sequencing. PCR products generated with this range of products are blunt ended.
PCRBIO VeriFi™ Polymerase enables high fidelity PCR of a wide range of targets and fragment sizes with minimal or no optimisation required. The enzyme is provided with an advanced buffer system including both dNTPs and Mg. For the most difficult templates, such as highly GC-rich sequences or those with complex secondary structures, users have the option of adding the VeriMax Enhancer to the reaction mix for improved PCR performance.
PCRBIO VeriFi™ Polymerase is also available as a convenient 2x ready mix containing all reaction components apart from primers and template. Our 2x red mix contains a red dye suitable for direct loading and tracking during agarose gel electrophoresis.
Applications
- High fidelity PCR
- Long range PCR
- Site-directed mutagenesis
- Cloning
- Sequencing
Storage
On arrival, products should be stored between -30 and -15°C. If stored correctly the kit will retain full activity for 12 months.
Figure 1. Versatility across a broad range of amplicon lengths
PCRBIO VeriFi™ Polymerase amplifies the range of fragment lengths indicated with high yield and specificity. The starting template amount is 4-30ng of mouse or human genomic DNA, diluted 1.5 to 3 fold. GC content ranges from 37-55%. L: PCRBIO Ladder II.
Figure 2. Increased success rates with complex templates
Amplification of a 17.5kb fragment of the HBB gene. The starting template amount is 150ng (A and C) and 30ng (B and D) of human genomic DNA, diluted 2 fold. A 2-step PCR protocol was used with amplification at 72°C (A and B) or 68°C (C and D). GC content is 37%. PCRBIO VeriFi™ Polymerase amplifies long fragments with yields comparable to Takara PrimeSTAR GXL DNA Polymerase. L: PCRBIO Ladder II.
Figure 3. Amplification of targets with high sensitivity and specificity compared to leading competitors
Amplification of a 1.0kb fragment of the GAPDH gene with different starting template amounts of mouse genomic DNA. A: 20ng, B: 3.2ng, C: 0.5ng, D: 0.08ng. GC content is 51%. L: PCRBIO Ladder IV. The reactions were set up following manufacturers’ recommendations. Cycling conditions were 95°C 2 min, then 30 cycles of 98°C 15 sec, 66°C 15 sec and 72°C 30 sec. PCRBIO VeriFi™ Polymerase displays greater sensitivity and specificity compared to leading competitors.
Available sizes:
| PB10.42-01 | 1 x 50μL | 100 Units |
| PB10.42-05 | 1 x 250μL | 500 Units |
Specifications
| PCRBIO VeriFi™ Polymerase (2u/μL) |
1 x 50μL | 100 Units 1 x 250μL | 500 Units |
| 5x PCRBIO VeriFi™ Buffer |
1 x 1.7mL | 100 Units 3 x 1.7mL | 500 Units |
| 10x VeriMax Enhancer |
1 x 1.7mL | 100 Units 2 x 1.7mL | 100 Units |
| Reaction Volume |
50μL |
Related products
Art No | PB10.44
VeriFi™ Mix Red - Long range and high fidelity PCR
from 170.00 CHF