PCRBIO Ultra Polymerase - Long range and "difficult" PCR (Incl. dNTPs)

Art No | PB10.31

PCRBIO Ultra Polymerase - Long range and "difficult" PCR (Incl. dNTPs)

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from 190.00 CHF

PCRBIO Ultra Polymerase has been engineered for the amplification of extremely difficult templates.

Description

Proprietary modifications that enhance processivity together with advanced buffer chemistry and hot start technology deliver outstanding performance whether your template is GC or AT-rich, low in abundance or contains PCR inhibitors.

Increased PCR success rates with difficult templates
Exhibits high tolerance to PCR inhibitors
Efficient specific amplification from complex templates including GC-rich sequences (up to 80%)
Antibody-mediated hot start for unrivalled detection of low copy number templates
Advanced buffer chemistry including Mg and dNTPs
High yields under standard and fast PCR conditions
3 fold higher fidelity than Taq
Available as a convenient 2x ready mix with the option of a red dye for direct gel loading

PCRBIO Ultra Polymerase is a highly robust enzyme, designed for efficient and reliable amplification of challenging and complex targets, even under difficult conditions such as the presence of inhibitors. The enzyme and buffer system have been developed to give superior PCR performance and higher success rates on a broad range of templates, including complex genomic DNA and targets with a high GC content (up to 80% GC).

PCRBIO Ultra Polymerase exhibits a high tolerance to PCR inhibitors making it the ideal choice for colony and crude sample PCR. PCR products generated are A-tailed and may be cloned into TA cloning vectors. The enzyme is supplied with an optimised buffer containing dNTPs and MgCl2, and is stable at 25°C for 4 weeks.

Our antibody-mediated hot start formulation prevents the formation of primer dimers and non-specific products, enabling specific and sensitive amplification from low copy number target sequences. For added convenience PCRBIO Ultra Polymerase is also available as a 2x ready mix containing all reaction components except primers and template. PCRBIO Ultra Mix Red contains a preloaded red dye suitable for direct loading and tracking during agarose gel electrophoresis.

Applications

Difficult PCR - GC and AT-rich DNA
Low copy template detection
Crude sample PCR
Colony PCR
Long range PCR
Multiplex PCR
TA cloning
Next generation sequencing

Storage

On arrival, products should be stored between -30 and -15°C. If stored correctly the kit will retain full activity for 12 months.

Material Safety Data Sheet (PDF)

Product manual (PDF)

FAQs

Figure 1. Long PCR products visualised on agarose gel

Amplification of 0.8kb, 1.0kb, 1.8kb, and 2.7kb fragments of the GAPDH gene, a 4.6kb fragment of the RBL15 gene, and a 5.9kb fragment of the MYH6 gene. The starting template amount is 20ng (50ng for the 5.9kb fragment) of mouse genomic DNA and is diluted 2 to 5 fold. PCRBIO Ultra Polymerase amplifies the range of fragment lengths indicated with high yield and specificity.

Figure 2. GC rich products visualised on agarose gel

Amplification of 0.5kb (P1) and 0.6kb (P2) fragments of the ATXN2 gene with GC contents of 69% and 71%, respectively, using 20ng of mouse genomic DNA
as template and a range of annealing temperatures from 67°C to 60°C (B-E). PCRBIO Ultra Polymerase efficiently amplifies GC rich templates >65% GC and is recommended for templates up to 80% GC.