Art No | PB71.22-05
NGSBIO DNA Standards for Illumina® | 500 x 20μL Reactions
370.00 CHF
The key to sequencing success.
Description
The NGSBIO Library Quant Kit offers a reliable qPCR-based method for the quantification of libraries prepared for Illumina NGS systems. The kit includes 5 DNA standards, primers specific to the P5 and P7 Illumina adapter sequences and qPCRBIO SyGreen® Mix. The advanced qPCR buffer system has been developed using PCR Biosystem's high-throughput smart screen technology to ensure efficient amplification of all your libraries, including those that are GC or AT-rich.
The kit is also supplied with a convenient library dilution buffer. This kit can also be used in combination with VeriFi™ Library Amplification Mix.
- Easy-to-see blue mix for greater pipetting precision and accuracy
- Uses qPCR to accurately and rapidly quantify a library prior to sequencing
- Gives consistent library quantification across a wide range of sample types, concentrations, fragment sizes and GC content
- Uses a single extension time for all libraries
- Suitable for manual and automated workflows
- Allows specific quantification of only DNA molecules that can be sequenced by NGS
- Uses antibody-mediated hot start technology to ensure all reactions start simultaneously
- Compatible with all Illumina instruments and qPCR platforms
- Available as an easy-to-see blue qPCR mix
Accurate Library Quantification
qPCR is considered the best method for quantifying NGS libraries as it only measures adapter-bound molecules that can be used as templates for library amplification and cluster generation. The NGSBIO Library Quant Kit enables highly accurate quantification crucial for optimal cluster densities and greater sequencing efficiency.
Library quantification can be used at any time after adapter ligation and should always be used prior to cluster generation. For easy and accurate data analysis we recommend using the NGSBIO Library Quantification Tool. Overestimating library concentration can result in insufficient cluster density and underestimating library concentration can result in a high, saturating cluster density. To learn more about optimal cluster density, consult the instructions of your Illumina machine.
Specifications
| Reaction Volume |
20μL |
| Storage |
On arrival, products should be stored between -30 and -20 °C. If stored correctly the kit will retain full until the indicated expiry date. |
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