IsoFast™ Bst Polymerase with Dye

Art No | PB80.11

IsoFast™ Bst Polymerase with Dye

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from 140.00 CHF

The fastest way to your results.

Description

IsoFast® Bst Polymerase with Dye from PCR Biosystems is the ultimate solution for rapid, robust, and reliable isothermal amplification. This innovative enzyme is engineered for exceptional speed, allowing you to achieve results in a fraction of the time.

Pre-mixed with an inert dye, it offers the convenience of direct gel loading, eliminating a post-reaction step and streamlining your workflow. Ideal for LAMP, NASBA, and other isothermal applications, IsoFast® Bst Polymerase with Dye delivers high yields and superior performance even with challenging templates.

IsoFast® Bst Polymerase is a recombinant form of the large fragment of Bst DNA polymerase, containing 5′-3′ polymerase activity and strand displacement activity.

The enzyme offers fast amplification and strong strand displacement capabilities, making it ideal for nucleic acid amplification methods such as whole genome amplification, multiple displacement amplification and isothermal amplification.

Has strand-displacing 5′-3′ polymerase activity
Lacks 5′-3′ exonuclease activity
DNA synthesis is performed at a constant temperature
Operates over a broad temperature range, with an optimum of 65ºC
Gives rapid and consistent amplification across a wide range of templates
Supplied with an advanced 2-part buffer system for higher yields under difficult conditions
30 minute protocol
Flexible formats with and without fluorescent dye
Also available as a 2x mix and as a dual enzyme 1-step system for RNA amplification
Glycerol-free enzyme

Applications

Whole genome amplification
Multiple displacement amplification
Isothermal amplification
Loop mediated isothermal amplification (LAMP)
Molecular diagnostics
Field diagnostics

With its exceptional speed and robust activity, IsoFast® Bst Polymerase allows you to achieve fast and accurate results without the need for a thermal cycler.

Exceptional Speed: Achieve faster amplification and reduce your reaction times.
High Yields: Get high-quality results with superior DNA synthesis activity.
Enhanced Thermostability: Maintain optimal performance over a wide range of temperatures.
Versatile: Perfect for a wide variety of isothermal applications.

IsoFast® Bst Polymerase is a recombinant protein expressed in E. coli and represents the large fragment of Geobacillus stearothermophilus (formerly known as Bacillus stearothermophilus) DNA Polymerase. This portion of the protein catalyses the 5′-3′ synthesis of DNA and has strand displacement activity but does not contain the 5’-3’ exonuclease domain.

Strong strand displacement

Strand displacement refers to the ability of an enzyme to dissociate the hydrogen bond of double stranded template DNA encountered downstream, essentially unzipping the DNA as the complementary strand is synthesised. IsoFast® Bst Polymerase displays strong strand displacement activity and is suitable for amplification methods including whole genome amplification, multiple displacement amplification and isothermal amplification. DNA synthesis is performed at a constant temperature, and we recommend running the reaction at 65 ºC. However IsoFast® Bst Polymerase works well over a broad temperature range (55 ºC to 70 ºC, giving a wider range of reaction conditions in order to optimise primer annealing and strand displacement. The enzyme is heat inactivated at 80 ºC.

Rapid and consistent results

Designed for fast amplification speed, IsoFast® Bst Polymerase gives rapid and consistent results across different target sequences. The enzyme is provided with an advanced 2-part buffer system to ensure higher yield and performance even under difficult conditions. Amplification can be detected in a number of ways including real-time fluorescence detection and end-point visualisation.

Format flexibility

For added convenience the enzyme is available with separate fluorescent dye (enabling real-time detection with any qPCR thermocycler), and in a 2x mix format for those looking for reduced setup times. The enzyme gives consistent results regardless of the format chosen.

This product is not suitable for PCR.

Material Safety Data Sheet (PDF)

Product manual (PDF)

FAQs

PCRBIO Tm Calculator

Isothermal Amplification

Isothermal amplification refers to a range of methods that rely on an alternative approach to amplification that is not limited by the requirement for thermal cycling.

Instead of denaturing DNA by using high temperatures, isothermal amplification takes advantage of DNA polymerases that have high strand displacement capabilities, for example Bst or Phi29 DNA polymerases. These enzymes have the ability to ‘unzip’ DNA as they synthesise complementary strands, removing the need for thermocycler equipment as the reaction is carried out at a one temperature. Isothermal methods making use of strand displacing DNA polymerases include multiple displacement amplification, whole genome amplification and loop mediated isothermal amplification or LAMP.

PCR Biosystems’ IsoFast™ Bst range of products is based on the large fragment of Bst DNA polymerase and offers rapid and sensitive amplification of DNA and RNA targets at a constant temperature of around 65°C.

Supplier

PCR Biosystems
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Specifications

IsoFast Bst Polymerase 8U/µL	1600 Units: 1 x 200µL 8000 Units: 1 x 1mL
10x IsoFast Buffer A	1600 Units: 1 x 500µL 8000 Units: 2 x 1.25mL
5x IsoFast Buffer B	1600 Units: 1 x 1mL 8000 Units: 3 x 1.7mL
20x Fluorescent Dye	1600 Units: 2 x 125µL 8000 Units: 2 x 625µL
Reaction Volume	25µL