UltraScript 2.0 Reverse Transcriptase

Art No | PB30.33

UltraScript 2.0 Reverse Transcriptase

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from 240.00 CHF

UltraScript 2.0 Reverse Transcriptase is a highly thermostable reverse transcriptase engineered for superior cDNA synthesis speed, yield and representation of the most challenging RNA sample types.

Description

This modified MMLV reverse transcriptase can be used with reaction temperatures of over 55°C, giving improved specificity, higher cDNA yields and more full length cDNA product.

Highly thermostable reverse transcriptase 55°C to 65°C and above
Advanced RNase inhibitor
High cDNA yields from as little as 20pg total RNA
Accurate reverse transcription of GC-rich and highly structured transcripts
High sensitivity for challenging templates
Reduced RNase H activity
Available as a stand-alone enzyme with buffer, a cDNA synthesis kit with premixed oligos and a cDNA synthesis kit with separate oligos

The enhanced thermostability of UltraScript 2.0 Reverse Transcriptase (RTase) allows reaction temperatures of over 55°C to be used, giving improved specificity, higher cDNA yields, and more full length cDNA product. The enzyme remains partially active even up to 90°C and is designed for efficient reverse transcription of the most difficult RNA templates, including GC-rich and highly structured transcripts.

UltraScript 2.0 RTase is designed for efficient cDNA synthesis from a broad range of RNA concentrations. UltraScript 2.0 kits can be used with 20pg to 3.5μg total RNA or oligo(dT) purified mRNA. The RTase is blended with an advanced RNase inhibitor preventing degradation of RNA by contaminating RNase.

UltraScript 2.0 RTase is available in three convenient formats, each with dNTPs, MgCl₂, and RNase inhibitor included. Our stand-alone enzyme with 5x buffer gives users the flexibility to define their own priming strategy and offers exceptional performance with gene-specific primers, oligo(dT) and random hexamers.

The 2-tube UltraScript 2.0 cDNA Synthesis Kit contains all the components required for fast, reliable and unbiased cDNA synthesis, including an optimised blend of anchored oligo(dT) and random hexamers. UltraScript 2.0 cDNA Synthesis Kit Separate Oligos provides anchored oligo(dT) and random hexamers in separate tubes, for user optimisation depending on the type of analysis needed.

Applications

cDNA synthesis for PCR analysis, cloning, cDNA library preparation and Next Generation Sequencing
Viral RNA targets
miRNA targets
Efficient synthesis from total RNA or poly(A)+ RNA

Storage

The kit should be stored between -30°C and -15°C. Avoid prolonged exposure to light. If stored correctly the kit will retain full activity for 12 months. The kit can be stored at 4°C for 1 month. The kit can go through 30 freeze/thaw cycles with no loss of activity.

Material Safety Data Sheet (PDF)

Product manual (PDF)

FAQs

Figure 1.

UltraScript 2.0 Reverse Transcriptase maintains
higher specific activity at elevated temperatures when compared to competing products and our original UltraScript Reverse Transcriptase. Specific activity is measured at the given incubation temperatures using an RT-qPCR assay.

Figure 2.

Mouse liver total RNA was reverse transcribed using UltraScript 2.0 Reverse Transcriptase, followed by amplification of G-Act cDNA with qPCRBIO SyGreen Mix. UltraScript 2.0 Reverse Transcriptase can transcribe up to 3.5µg of RNA while retaining a linear response.

Figure 3.

Mouse liver total RNA was reverse transcribed using UltraScript 2.0 Reverse Transcriptase, followed by amplification of G-Act cDNA using qPCRBIO SyGreen Mix. Up to 65°C, UltraScript 2.0 Reverse Transcriptase shows little change in yield with ΔCt values within ± 1 Ct range (3b), and remains partially active up to 90°C (3a and 3b).

Figure 4.

UltraScript 2.0 Reverse Transcriptase gives similar amounts of product across a wide range of temperatures in endpoint RT-PCR. Mouse reference RNA was reverse transcribed using UltraScript 2.0 Reverse Transcriptase. G-Act cDNA was amplified using qPCRBIO SyGreen Mix and visualised on EtBr 1% agarose gel.

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