EchoLUTION Tissue RNA Kit – Witec AG

Art No | 011-TISSUE-RNA
from 120.00 CHF

For single-step purification of total RNA from mammalian tissue samples (fresh, frozen, or stabilized)

Description

  • Convenience and speed:
    Single-step purification, saving up to 60 % of processing time
  • High compatibility:
    Suitable for extraction of total RNA from fresh frozen or stabilized mammalian tissues, including challenging tissues such as muscle
  • High sensitivity:
    Highly pure RNA free of contaminants and inhibitors
  • Reliable results:
    High purity and competitive RNA integrity perfectly suited for downstream applications such as RT-qPCR and RNA-seq
  • Sustainability:
    Less hazardous reagents - quality RNA without TRIzol/chloroform

Specification at a glance

Icon-Sample-input

Sample input: up to 10 mg of tissue (fresh-frozen, stabilized), depending on tissue type

Icon-Processing

Processing: Manual through centrifugation/(semi)automated with liquid handler/robot

Icon-Expected-yield

Expected yield: Up to 8–14 µg
(depending on tissue type and input)

Icon-RNA-quality

RNA quality: RIN 6.5–9

Icon-Output-volume

Output volume: 80 µL

Icon-Downstream-compatibililty

Downstream compatibility: RT-PCR, RT-qPCR, RNA-seq

High-quality RNA with no phenol/chloroform

The EchoLUTION Tissue RNA Kit is intended for easy, rapid, and efficient RNA extraction from mammalian tissue samples (liver, muscle, spleen, lung, and nervous tissues). Our EchoLUTION technology allows the extraction of nucleic acids in a single step after sample homogenization and ultra-fast lysis.

The excellent yield and purity of total RNA obtained with the EchoLUTION Tissue RNA Kit makes it perfectly suited for downstream applications such as RT-qPCR and RNA-seq. Like this, it gives researchers and scientists in drug discovery, sequencing, and/or basic research, such as oncology, immunology, and transcriptomics a solution at hand.

The EchoLUTION Technology

The EchoLUTIONTM Tissue RNA Kit provides:

Convenience and speed

Single-step purification allows complete extraction of 96 samples within 35 minutes.

High compatibility

Suitable for extraction of total RNA from fresh-frozen or stabilized mammalian tissues, including challenging tissues such as muscle

High Sensitivity

Highly pure total RNA free of contaminants and inhibitors

Sustainability

Up to 54 % less plastic consumption compared to other extraction methods and eliminating the need to use TRIzol®/chloroform.

The workflow: faster and fewer steps

The single-step purification technology from BioEcho reduces the hands-on time and total number of steps. Combined with an ultra-fast lysis, it allows the RNA extraction of 6 samples to be completed within 25 minutes (spin column format) or a 96-well plate within 35 minutes. The plate kit can be used manually, as well as in semi- or fully automated systems.

EchoLUTION-workflow
EchoLUTION-workflow2

Supplier

BioEcho Life Sciences GmbH
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Specifications

50 rxn Kit content:
  • Lysis Buffer Tissue RNA concentrate (LB)  (112.5 mg)
  • Lysis Solution Tissue RNA (LS) (7.5 mL)
  • Clearing Solution Tissue RNA (CS) (1.2 mL)
  • DNase Tissue (D) (260 µL)
  • Reaction Buffer DNase Tissue (RB) (110 µL)
  • Low-TE Buffer (T) (1.2 mL)
  • Spin Columns Tissue RNA (50)
250 rxn Kit content:
  • Lysis Buffer Tissue RNA concentrate (LB) (2 x 270 mg)
  • Lysis Solution Tissue RNA (LS) (2 x 19.5 mL)
  • Clearing Solution Tissue RNA (CS) (6 mL)
  • DNase Tissue (D) (2 x 650 µL)
  • Reaction Buffer DNase Tissue (RB) (550 µL)
  • Low-TE Buffer (T) (1.2 mL)
  • Spin Columns Tissue RNA (250)
2 x 96 rxn Kit content:
  • Lysis Buffer Tissue RNA concentrate (LB) (412.5 mg)
  • Lysis Solution Tissue RNA (LS) (30 mL)
  • Clearing Solution Tissue RNA (CS) (4.35 mL)
  • DNase Tissue (D)(1.1 mL)
  • Reaction Buffer DNase Tissue (RB) (500 µL)
  • Low-TE Buffer (T) (1.2 mL)
  • Purification Plate 96 Type 4 (2 plates)
  • Elution Plate 96 Type  (2 plates)
  • Adhesive Foil (2 foils)
8 x 96 rxn Kit content:
  • Lysis Buffer Tissue RNA concentrate (LB)  (4 x 412.5 mg)
  • Lysis Solution Tissue RNA (LS) (4 x 30 mL)
  • Clearing Solution Tissue RNA (CS)  (18 mL)
  • DNase Tissue (D)  (4 x 1.1 mL)
  • Reaction Buffer DNase Tissue (RB) (4 x 500 µL)
  • Low-TE Buffer (T) (2 x 1.2 mL)
  • Purification Plate 96 Type 4 (8 plates)
  • Elution Plate 96 Type 1 (8 plates)
  • Adhesive Foil (8 foils)

Accessories

Art No | 050-001-001
BioEcho Cap Puncher

For efficient handling of EchoLUTION Spin Columns.

15.00 CHF
15.00 CHF

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