DNBSEQ-G50RS NGS Genetic Sequencer – Witec AG

Art No | 900-000182-00
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request only

DNBSEQ™ technology, an Error-free Nanoarray Sequencing Technology, delivers multiple read lengths and optimized sequencing time, enabling you to complete sequencing in a short period of time with high quality yield in standard FASTQ format.

Description

DNBSEQ-G50 is a sequencer designed for lower throughput and faster turnaround times.  The  DNBSEQ-G50 adopts two colour chemistry and DNBseq to support rapid sequencing in various different read modes.

  • Zero accumulation of error in amplification, with high-precision base read-through
  • 4 types of reads are available. Running the PE100 process at full load takes only 48 hours
  • Flexible data yield from 10Gb to 60Gb
  • Sequencing time of 10 hours to 64 hours
  • Flexible: Various read length options & compatible with different sample types
  • Accessible: Cost-effective & adaptable in different operating environment
  • Intelligent: Fully-automated operation & integrated analysis software

 

Performance Parameters

Slide No.: 1
Slide Type: FCS
Lane No./Slide: 1
Effective Reads/Slide*: 300M
Read lengths: SE50 | SE100 | PE50 | PE100

*The maximum number of effective reads are based on the sequencing of an internal standard library. Actual output may vary depending on sample type and library preparation method.

 

FLOW CELL READ LENGTH DATA OUTPUT Q30% TAT
FCS-100M SE100 10G >80% ~10h
PE100 20G >80% ~28h
PE150 30G >75% ~41h
FCL-500M SE50 25G >80% ~11h
SE100 50G >80% ~18h
PE100 100G >80% ~45h
PE150 150G >75% ~64h

 

 

DNBSEQ-G50 NGS Sequencer detailed

Non-accumulative amplification errors
High precision base reading

By employing DNA Nanoballs (DNB) technology and its special linear amplification method, DNBSEQ-G50 effectively carries out high-rate amplification, while ensuring a reduced error rate.

Moreover, its nanochip spot design allows for signal enhancement while ensuring sequencing accuracy without signal interference. DNBSEQ-G50 uses improved combinatorial Probe-Anchor Synthesis (cPAS) technology to incorporate a fluorescent probe to a DNA anchor on the DNB. A high-resolution imaging system is then used to collect, read and identify optical signals.

This sequencing system not only avoids highly repetitive single-base reading errors caused by common physical signal errors, but it also produces high quality and high accuracy sample sequencing information.

MGI’ S PROPRIETARY DNBSEQTM TECHNOLOGY

INCREASED ACCURACY
No PCR amplification required. Our unique Rolling Circle Replication (RCR) technology employed in DNBSEQTM library construction eliminates errors associated with PCR. Only the original template DNA is used to generate copies and therefore amplification errors do not accumulate, resulting in greater accuracy for detection of significant mutations such as lndels and SNPs.

DECREASED DUPLICATES
Optimized Patterned Array ensures that only one single DNB is attached at each spot, which results in greater saturation of DNB on the Flow Cell with unprecedented uniformity. This enables an industry-leading with reduced duplicate rate.

REDUCED INDEX HOPPING
MGI platform’s unique library preparation method and RCR amplification results in much lower index hopping rates compared with other platf orms, at a rate of 0.0001%~0.0004%.

DNB Technology

Supplier

MGI Tech Co. Ltd.
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Specifications

DNBSEQ-G50RS

for research only

Touch Screen
  • Size: 10inches
  • Resolution: 1280×800 (60 Hz)
Maximum Sound Pressure:
  • 70dB
Shell Protection Grade
  • IPX0
Control Computer Configurations
  • CPU: i7-4790
  • Internal Storage: 16 GB RAM
  • HDD: 1TB
  • SSD: 250GB
  • Operating System: Windows 10 IOT (64-bit version)
Operating Environment Requirements
  • Temperature: 19~25℃
  • Relative Humidity: 20%RH~80%RH, non-condensing
  • Atmospheric Pressure: 70kPa~106kPa
  • Waterproof Rating: IPX0
Power | Dimensions
  • Type: 100~240V, 50/60Hz
  • Rated Power: 900VA
  • Dimensions: Length 654mm, Width 489mm, Height 545mm
  • Net Weight: 85kg

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