DNBSEQ™ technology, an Error-free Nanoarray Sequencing Technology, delivers multiple read lengths and optimized sequencing time, enabling you to complete sequencing in a short period of time with high quality yield in standard FASTQ format.
DNBSEQ-G50 is a sequencer designed for lower throughput and faster turnaround times. The DNBSEQ-G50 adopts two colour chemistry and DNBseq to support rapid sequencing in various different read modes.
- Zero accumulation of error in amplification, with high-precision base read-through
- 4 types of reads are available. Running the PE100 process at full load takes only 48 hours
- Flexible data yield from 10Gb to 60Gb
- Sequencing time of 10 hours to 64 hours
- Flexible: Various read length options & compatible with different sample types
- Accessible: Cost-effective & adaptable in different operating environment
- Intelligent: Fully-automated operation & integrated analysis software
Slide No.: 1
Slide Type: FCS
Lane No./Slide: 1
Effective Reads/Slide*: 300M
Read lengths: SE50 | SE100 | PE50 | PE100
*The maximum number of effective reads are based on the sequencing of an internal standard library. Actual output may vary depending on sample type and library preparation method.
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Non-accumulative amplification errors
High precision base reading
By employing DNA Nanoballs (DNB) technology and its special linear amplification method, DNBSEQ-G50 effectively carries out high-rate amplification, while ensuring a reduced error rate.
Moreover, its nanochip spot design allows for signal enhancement while ensuring sequencing accuracy without signal interference. DNBSEQ-G50 uses improved combinatorial Probe-Anchor Synthesis (cPAS) technology to incorporate a fluorescent probe to a DNA anchor on the DNB. A high-resolution imaging system is then used to collect, read and identify optical signals.
This sequencing system not only avoids highly repetitive single-base reading errors caused by common physical signal errors, but it also produces high quality and high accuracy sample sequencing information.
MGI’ S PROPRIETARY DNBSEQTM TECHNOLOGY
No PCR amplification required. Our unique Rolling Circle Replication (RCR) technology employed in DNBSEQTM library construction eliminates errors associated with PCR. Only the original template DNA is used to generate copies and therefore amplification errors do not accumulate, resulting in greater accuracy for detection of significant mutations such as lndels and SNPs.
Optimized Patterned Array ensures that only one single DNB is attached at each spot, which results in greater saturation of DNB on the Flow Cell with unprecedented uniformity. This enables an industry-leading with reduced duplicate rate.
REDUCED INDEX HOPPING
MGI platform’s unique library preparation method and RCR amplification results in much lower index hopping rates compared with other platf orms, at a rate of 0.0001%~0.0004%.
SupplierMGI Tech Co. Ltd.
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