Nikon Objective CFI Plan Apochromat Lambda D 2X

Art No | MRD70020

Nikon Objective CFI Plan Apochromat Lambda D 2X

1'709.00 CHF

Brochure

Capture Every Detail of Biological Phenomena

Description

The newly developed CFI Plan Apochromat Lambda D is a high-performance objective series optimized for digital solutions that are essential for future life science research.

The Lambda D series objectives deliver high image quality across the large field of view of 25 mm and chromatic aberration correction over a wide wavelength range. They improve the accuracy of quantitative analysis and realize highly reliable data acquisition.

Primary Technique: Brightfield
Recommended for best results: Brightfield | FL Visible | FL NIR
Immersion: Air
Magnification: 2x
Numerical Aperture: 0.1
Working Distance: 8.5
Cover Glass Thickness: 0-0.17
Application Area: Clinical/Laboratory | Confocal | Research

Bright and Clear over a Large Field of View

Image flatness has been improved by adopting a newly-developed high-refractive index glass and correcting field curvature in the oil immersion objectives. Bright and clear images can be obtained right up to the edge of the 25 mm field of view.

This results in:

Improvement accuracy of light intensity measurement
Generation of seamless macro images in a short time
Acquisition of a wide range of life phenomena with a single image
Enhancement of high-content screening throughput

lambda-d_Bright and Clear over a Large Field of View

Reduced light intensity deterioration at the periphery of the field of view

The Lambda D series objectives minimize light intensity deterioration at the periphery of the field of view. This allows (DAPI stained) cell nuclei at the edges of the image to be observed brightly, even with confocal imaging.

lambdad_Lambda2a_20c1ad2fb3e82075627c86561425a107

With conventional objective

BPAE cells; Label: GFP, MitoTracker Red, DAPI; Microscope: AX R; Objective: CFI Plan Apochromat Lambda D 60X Oil

lambdad_Lambda2b_20c1ad2fb3e82075627c86561425a107

With Lambda D objective

BPAE cells; Label: GFP, MitoTracker Red, DAPI; Microscope: AX R; Objective: CFI Plan Apochromat Lambda D 60X Oil

High-precision image stitching

Since image acquisition time can be reduced thanks to a large 25 mm diagonal field of view and superior image quality up to the image periphery, seamless, high-quality stitched images can be efficiently obtained.

Mouse kidney; Label: Alexa 488 WGA, Alexa 568 Phalloidin, DAPI; Camera: DS-Qi2; Objective: CFI Plan Apochromat Lambda D 10X

The Endless Pursuit of High Resolution

Nikon’s technology processes the edges of lens elements to their minimum thickness. This maximizes the use of light rays passing through the lens periphery, thereby increasing the numerical aperture and delivering high resolution of fine structures.

A clear fluorescent image with a high S/N ratio can be obtained.

High resolution images can be captured with a large field of view.

lambda-d_high-signal-to-noise-ratio-images

Mouse intestine; Label: Alexa 488, Alexa 633, Microscope: AX R; Objective: CFI Plan Apochromat Lambda D 100X Oil

Mouse cerebral nerve (MIP); Label: GFP; Z range: 165.5 μm; Z step: 0.147 μm; Microscope: AX R; Objective: CFI Plan Apochromat Lambda D 100X Oil

Gastric wall; Label: SMA antibody staining; Camera: Digital Sight 10; Objective: CFI Plan Apochromat Lambda D 40XC; Image courtesy of Nichirei Biosciences Inc.

Overview Objectives (PDF)

Nikon Objective Selector