Art No | TCP-96WELL
96-well Detachable Flat Plates, sterile
from 211.00 CHF
Designed to improve cell attachment
Description
Poly-D-Lysine serves as a positively charged extracellular matrix, fostering nonspecific cell attachment. Upon application to solid-phase culture surfaces, it heightens the electrostatic interaction between negatively charged ions on the cell membrane surface and positively charged ions on the culture surface. This enhancement bolsters cell attachment rates in serum-free or low-serum culture settings and reinforces the absorption of serum proteins and extracellular matrix proteins onto the culture surface.
The product surface is pre-coated with Poly-D-lysine, which facilitates the attachment growth, proliferation and differentiation of cells that are difficult to culture, such as neurons, glial cells and transfected cell lines.
Available as:
- Poly-D-Lysine-coated culture plates (6-well, 12-well and 24-well)
- Poly-D-Lysine-coated culture dishes (35 mm, 60 mm and 90 mm)
APPLICATIONS
- Primary neuronal culture
- Culture of transfected cell lines with low adherence capability
- Neuronal differentiation and axonal culture
- Glial cell culture
- Culture of cells under serum-free or low-serum conditions
FEATURES
- Utilizing premium poly-D-lysine characterized by a molecular weight ranging from 75 to 150 kDa, boasting high viscosity and robust cell attachment properties
- Enhance the attachment, growth, and specialization of challenging cell types, such as neurons, in culture
- Synthetic poly-D-lysine aims to prevent the stimulation of biological activity resulting from the introduction of natural polymers, impurity proteins, and similar factors
- Following validation through diverse cell culture tests, the attachment rate of cells surpasses 90%, with the viability of attachment cells exceeding 95%
- Lot number of each package bag is printed to ensure quality traceability
- Sterilized by irradiation, SAL10-6, DNase/RNase-free, and non-pyrogenic
Inoculate PC-12 cells onto a poly-D-lysine-coated 24-well cell culture plate at a density of 5×105 cells/well.
After 24 hours, observe under a microscope:
The cell morphology appears normal, exhibiting an attachment rate surpassing 90% and the viability of attachment cells exceeding 95%.
Specifications
| Culture Plates |
|
| Culture Dishes |
|