Revolutionize Genomics with SMRT® Sequencing
Single Molecule, Real-Time Technology
Experience comprehensive genomics
Delivering valuable insights previously unavailable to the scientific community, the PacBio Sequel System provides an unmatched depth of genetic information through long-read sequencing and high consensus accuracy.
SEQUEL SYSTEM: high-throughput, cost-effective access to SMRT Sequencing
The new Sequel System is based on our proven Single Molecule, Real-Time (SMRT) technology and delivers about 7x more reads with 1 million zero-mode waveguides (ZMWs) per SMRT Cell. The Sequel System is ideal for projects such as rapidly and cost-effectively generating high-quality whole genome de novo assemblies.
This innovative sequencing system features automated reagent and SMRT Cell handling and an integrated software suite.
The Sequel System has two primary user access points:
- Sequel touchscreen interface (for initiating and monitoring runs)
- Sequel workdeck (for loading samples, SMRT Cells, reagents, and disposables)
The Sequel System is based on our proven Single Molecule, Real-Time (SMRT) Sequencing technology achieves:
- Generate up to 20 Gb per SMRT Cell with average read lengths up to 30 kb and achieve high consensus accuracies (>99.999%) for whole genome sequencing projects
- Generate up to 500,000 long, single-molecule reads with high fidelity (>99% accuracy) for amplicon and RNA sequencing projects
- >99.999% (QV50) consensus accuracy with data free of systematic errors
- With an efficient <1 day workflow
Advance genomics with long-read sequencing, enabled by Single Molecule, Real-Time (SMRT) Sequencing
SMRT Sequencing is built upon two key innovations: zero-mode waveguides (ZMWs) and phospholinked nucleotides. ZMWs allow light to illuminate only the bottom of a well in which a DNA polymerase/template complex is immobilized. Phospholinked nucleotides allow observation of the immobilized complex as the DNA polymerase produces a completely natural DNA strand.
The SMRT Sequencing advantage
SMRT Sequencing is ideal for a variety of research applications and offers many benefits, including:
- Longest average read lengths
- Highest consensus accuracy
- Uniform coverage
- Simultaneous epigenetic characterization
- Single-molecule resolution
Whole Genome Sequencing
- Human Whole Genome Sequencing
- Plant and Animal Whole Genome Sequencing
- Microbial Whole Genome Sequencing
- Structural Variation
Discovery is in the details
Generate gold-standard reference genomes and uncover the most complete view of genomic variation with PacBio long-read sequencing.
De novo Genome Assembly – no reference required
Whole genome sequencing for de novo assembly using Single Molecule, Real-Time (SMRT) Sequencing enables you to:
- Cultivate pan-genomes to characterize the complete genetic diversity within a species
- Develop population-specific reference genomes to drive precision medicine
- Assemble near-complete microbial genomes and their plasmids in a single experiment
- No-Amp Targeted Sequencing
- Targeted Sequencing for Human Biomedical Research
- Targeted Sequencing for Plant and Animal Sciences
- Targeted Sequencing for Microbiology and Infectious Disease Research
- HLA Sequencing
- BAC Sequencing
Focus in with access to the full spectrum of genetic variation
Accurately discover and detect all variant types even in the hardest to reach regions of the genome.
Single Molecule, Real-Time (SMRT) Sequencing and the Sequel System allow you to easily and cost effectively generate high-fidelity, long reads (>99% single-molecule read accuracy) from genes or regions of interest ranging in size from several hundred base pairs to 20 kb. With SMRT Sequencing you can:
- Detect all variant types including structural variants, rare SNPs, indels, copy number variation, microsatellites, haplotypes, and phased alleles
- Sequence through low complexity regions like repeat expansions, promoters, and flanking regions of transposable elements
- Resolve Viral Populations
- Characterize Microbial Communities
- Detect Somatic Variation
Characterize your population
Bacteria, viruses, and even cancer cells are often found in complex populations of closely related variants. These variants allow for rapid evolution in response to environmental conditions, immune pressures, or drug treatments. Distinguishing these co-existing species can require complex assembly, making the identification of closely related individuals within a mixture extremely challenging.
Resolve population complexity with accuracy and confidence
Single Molecule, Real-Time (SMRT) Sequencing generates the long reads, single-molecule resolution, and uniform coverage you need for the comprehensive characterization of heterogeneous samples and the identification of complex variation.
- Human RNA Sequencing
- RNA Sequencing for Plant and Animal Sciences
Discover full-length transcripts
Get a complete view of transcript isoform diversity with PacBio long-read sequencing.
Single Molecule, Real-Time (SMRT) Sequencing and Iso-Seq analysis allow you to generate full-length cDNA sequences — no assembly required — to characterize transcript isoforms within targeted genes or across an entire transcriptome so that you can easily and affordably:
- Discover new genes, transcripts and alternative splicing events
- Improve genome annotation to identify gene structure, regulatory elements,
and coding regions
- Increase the accuracy of RNA-seq quantification with isoform-level resolution
- Microbial Epigenetics
- Eukaryotic Epigenetics
The gateway to new biological discoveries
Epigenetic modifications affect a broad range of biological processes, including gene expression, host-pathogen interactions, environmental response, DNA damage, and DNA repair1. Epigenetics also play a significant role in the inheritance of traits from one generation to the next. While more than 20 types of epigenetic and DNA-damage modifications have been identified, most scientists have only been able to study one type, cytosine methylation, using indirect detection methods. The ability to directly detect these changes is critical for understanding the impact of DNA modification on a wide variety of natural processes and disease responses.
Characterize the epigenetic landscape of your genome
Single Molecule, Real-Time (SMRT) Sequencing directly detects epigenetic modifications by measuring kinetic variation during base incorporation. By capturing these modifications simultaneously with sequence data, this method eliminates the need for special sample preparation and additional sequencing.
PacBio Systems provide a direct view of epigenetic modification
- Characterize bacterial DNA modifications and methyltransferase recognition motifs
- Identify eukaryotic hyper- and hypo-methylated CpG islands to explore gene expression and regulation
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19ºC – 25ºC ± 2°C per hour
20% – 80%, noncondensing
HVAC capacity of up to 13,956 BTU (4090 Watts)
50 PSI (2,585 torr)
92.7 x 86.4 x 167.6 cm